魏荷琳 1王涛 2孙静静 2黄志强 2肖驿泽 2李军 3屠鹏飞4
作者信息
- 1. 泸州职业技术学院,四川泸州 646000
- 2. 北京中医药大学北京中医药研究院中医药现代研究中心,北京 102488
- 3. 北京大学医学部药学院天然与仿生药物国家重点实验室,北京 100191
- 4. 北京大学医学部药学院天然与仿生药物国家重点实验室,北京 100191||北京中医药大学北京中医药研究院中医药现代研究中心,北京 102488
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摘要
Abstract
Melicope pteleifolia is a plant belonging to the Melicope genus of the Rutaceae family.Known for a bitter taste and cold nature,its stems and tender branches with leaves possess properties of clearing heat,detoxifying,dispelling wind,and removing dampness and can be used to treat sore throat,malaria,jaundice hepatitis,rheumatic bone pain,eczema,dermatitis,and sores and ulcers.In this study,19 compounds were isolated from the chloroform and n-butanol extracts of M.pteleifolia leaves by using liquid chromatography-mass spectrometry(LC-MS)and proton nuclear magnetic resonance(1 H-NMR)-guided separation techniques.The compounds were identified as isoleptonol(1),leptaones B-E(2-5),friedelin(6),evodionol(7),ethyl p-hydroxybenzoate(8),litseachromolaevane A(9),quercetin-7,3',4'-trimethyl ether(10),kokusaginin(11),8-(1-hydroxyethyl)-5,6,7-trimethoxy-2,2-dimethyl-2H-1-benzopyran(12),ethyl p-hydroxycinnamate(13),3-hydroxy-9-methyl-6H-benzo[c]chromen-6-one(14),agrimonolide(15),7-hydroxycoumarin(16),scopoletin(17),isoscutellarein(18),and agrimonolide 6-O-glucoside(19).Among these,the new compounds included one chromene and four meroterpenoid(1-5).The anti-inflammatory activities of the newly identified compounds 1-5 were screened in vitro,showing that the five compounds(1-5)exhibited inhibitory effects on nitric oxide(NO)production in BV2 cells induced by lipopolysaccharide(LPS)/interferon(IFN)-γ,with IC50 values ranging from 12.25 to 36.48 μmol·L-1.关键词
三叉苦/化学成分/结构解析/抗炎活性Key words
Melicope pteleifolia/chemical component/structure elucidation/anti-inflammatory activity
