中国瓜菜2025，Vol.38Issue(9)：40-47,8.DOI:10.16861/j.cnki.zggc.2025.0258
西瓜中苹果酸代谢关键基因NADP-ME的克隆及功能分析
Cloning and functional analysis of NADP-ME,a key gene in malic acid metabolism in watermelon
摘要
Abstract
Organic acids in watermelon fruits are critical components influencing flavor quality,and elucidating the mo-lecular mechanism of malic acid synthesis and metabolism is of great significance.Through homologous sequence align-ment,two watermelon NADP-malic enzyme genes,designated as ClaNADP-ME3 and ClaNADP-ME4,were cloned.Quantitative real-time PCR(qRT-PCR)and high-performance liquid chromatography(HPLC)analysis revealed that the ex-pression level of ClaNADP-ME4 was negatively correlated the the malic acid content in watermelon.Subcellular localiza-tion analysis using GFP fusion proteins demonstrated that the ClaNADP-ME4 gene was distributed in both the cytoplasm and nucleus.Genetic transformation and overexpression of the ClaNADP-ME4 gene further conformed its function,dem-onstrating that this gene significantly reduced the malic acid content in watermelon fruits.In conclusion,the NADP-ME4 gene plays a role in promoting malic acid metabolism.These findings provide a new target for molecular breeding aimed at improving watermelon quality.关键词
西瓜/苹果酸/苹果酸酶/超表达Key words
Watermelon/Malic acid/NADP-malic enzyme/Overexpression分类
农业科技引用本文复制引用
董薇,杨从基,许守明,张洲,吴德峰,李军华..西瓜中苹果酸代谢关键基因NADP-ME的克隆及功能分析[J].中国瓜菜,2025,38(9):40-47,8.基金项目
河南省科技攻关项目(242102111085) （242102111085）
河南大学校级本科教学改革研究与实践项目(HDXJJG2022-013) （HDXJJG2022-013）
中原学者工作站项目(234400510004) （234400510004）
