中国中药杂志2025，Vol.50Issue(15)：4284-4292,9.DOI:10.19540/j.cnki.cjcmm.20250520.201
HPLC指纹图谱、特征图谱和多指标定量结合化学模式识别的不同基原鬼针草药材质量评价研究
Quality evaluation of Bidentis Herba derived from different original plants based on HPLC fingerprints,characteristic chromatograms,multi-component content determination combined with chemical pattern recognition
摘要
Abstract
This study established the HPLC fingerprints,characteristic chromatograms,and a multi-component content determination method for Bidens bipinnata and B.biternata.The chemical pattern recognition analysis was then employed to clarify the characteristic indexes of quality differences between the two original plants of Bidentis Herba,providing a reference for establishing the quality standards of Bidentis Herba.HPLC was launched on an Agilent Poroshell 120 EC-C18 chromatographic column(4.6 mm×250 mm,4 μm)by gradient elution with a mobile phase of 0.1％aqueous phosphoric acid-acetonitrile at a flow rate of 0.7 mL·min-1,detection wavelength of 270 nm,column temperature of 25℃,and an injection volume of 5 μL.The similarity between the fingerprints of 18 batches of Bidentis Herba samples and the common pattern(R)ranged from 0.572 to 0.933.A total of 23 chromatographic peaks were calibrated.Through comparison with the reference substances,six components(neochlorogenic acid,chlorogenic acid,isochlorogenic acid A,isochlorogenic acid B,rutin,and hyperoside)were identified and subjected to quantitative analysis.The characteristic fingerprints of B.bipinnata and B.biternata were calibrated with 20 and 17 characteristic peaks,respectively.Among them,peaks 8,9,22,and 23 were the characteristic peaks of B.bipinnata,and peak 7 was the characteristic peak of B.biternata,which can be used to distinguish the two original plants of Bidentis Herba.The relative standard deviation of the content of the above-mentioned six components ranged from 36％to 123％.The cluster analysis,principal component analysis,and orthogonal partial least squares-discriminant analysis(OPLS-DA)classified the 18 batches of Bidentis Herba samples into two categories.Additionally,through the analysis of variable importance in projection(VIP)under OPLS-DA,three characteristic indexes,rutin,isochlorogenic acid A,and isochlorogenic acid B,were identified.The analytical method established in this study can comprehensively evaluate the consistency of Bidentis Herba samples derived from different original plants,specifically identify the differential components between them,and effectively distinguish the two original plants of Bidentis Herba,providing a basis for the differentiation between different original plants and the quality control of Bidentis Herba.关键词
婆婆针/金盏银盘/指纹图谱/特征图谱/化学模式识别/含量测定/质量控制Key words
Bidens bipinnata/B.biternata/fingerprint/characteristic chromatogram/chemical pattern recognition/content deter-mination/quality control引用本文复制引用
石国丽,马云,申凤霞,杜汉文,刘从敏,魏瑞霞,李艳芳,范建伟,关永霞..HPLC指纹图谱、特征图谱和多指标定量结合化学模式识别的不同基原鬼针草药材质量评价研究[J].中国中药杂志,2025,50(15):4284-4292,9.基金项目
山东省重点研发计划(竞争性创新平台)项目(2024CXPT074) （竞争性创新平台）
