中国药房2025，Vol.36Issue(18)：2244-2249,6.DOI:10.6039/j.issn.1001-0408.2025.18.05
瑶药上山虎成分分析、指纹图谱建立及抗炎谱效关系研究
Study on component analysis,fingerprint establishment and anti-inflammatory spectrum-effect relationship of Yao ethnic medicine Pittosporum pauciflorum
摘要
Abstract
OBJECTIVE To analyze chemical components of Yao ethnic medicine Pittosporum pauciflorum,establish its fingerprint and investigate the spectrum-effect relationship of its anti-inflammatory effect.METHODS UHPLC-Q-Orbitrap-MS technology was used to analyze the chemical components of P.pauciflorum(batch S6).The fingerprints for 10 batches of P.pauciflorum from different producing areas in Guangxi Province(batches S1-S10)were established by HPLC,and similarity assessment and chemometric pattern recognition analysis were conducted.RAW264.7 inflammatory cell model was induced by lipopolysaccharide,and the anti-inflammatory activity of P.pauciflorum was investigated.Using inhibition rates of nitric oxide(NO),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and IL-1β as efficacy indicators,grey relational analysis and partial least squares regression analysis were adopted to evaluate the spectrum-effect relationship of the anti-inflammatory effect of P.pauciflorum.RESULTS There were 60 chemical components,including flavonoids,phenolic acids,lipids,etc.,identified in P.pauciflorum.The fingerprints for 10 batches of P.pauciflorum showed 14 common peaks,with similarity values ranging from 0.883 to 0.991.Three common peaks were assigned neochlorogenic acid(peak 5),chlorogenic acid(peak 7),and syringaldehyde(peak 10).The classification results of the systematic clustering analysis and the principal component analysis were basically consistent.Batches S1 to S10 of P.pauciflorum significantly reduced the levels of NO,TNF-α,IL-6(except for batch S5)and IL-1β in the cell supernatant(P＜0.05 or P＜0.01).Inhibition rates of above inflammatory indexes were 10.26%-39.96%,14.96%-31.36%,1.38%-21.27%,18.54%-28.00%,respectively.The contents of neochlorogenic acid,syringaldehyde,as well as the components corresponding to peaks 1,3,9,12 and 14,exhibited a strong correlation with the anti-inflammatory effects of P.pauciflorum.CONCLUSIONS The present study has analyzed the chemical components of P.pauciflorum and established HPLC fingerprints for 10 batches of P.pauciflorum.Each batch of medicinal herbs demonstrates certain anti-inflammatory activities,among which neochlorogenic acid,syringaldehyde,and the components corresponding to peaks 1,3,9,12 and 14 are likely to be the active anti-inflammatory components.关键词
瑶药/上山虎/化学成分/指纹图谱/抗炎作用/谱效关系/广西不同产地Key words
Yao ethnic medicine/Pittosporum pauciflorum/chemical components/fingerprint/anti-inflammatory effect/spectrum-effect relationship/different producing areas in Guangxi Province分类
医药卫生引用本文复制引用
秦丹,傅鹏,曹家杰,唐清晨,高洁..瑶药上山虎成分分析、指纹图谱建立及抗炎谱效关系研究[J].中国药房,2025,36(18):2244-2249,6.基金项目
国家重点研发计划课题(No.2019YFC1712304) （No.2019YFC1712304）
广西壮族自治区重点实验室培育基地建设项目——广西壮瑶药重点实验室(No.桂科基字[2014]32号) （No.桂科基字[2014]32号）
广西协同创新中心建设项目——壮瑶药协同创新中心(No.桂教科研[2014]13号) （No.桂教科研[2014]13号）
广西壮族自治区工程研究中心建设项目——广西壮族自治区民族药资源与应用工程研究中心(No.桂发改高技函[2020]2605号) （No.桂发改高技函[2020]2605号）
