中国中药杂志2025，Vol.50Issue(15)：4333-4341,9.DOI:10.19540/j.cnki.cjcmm.20250421.402
六君子丸对脾气虚证大鼠肠道菌群的影响及机制研究
Effect and mechanism of Liujunzi Pills on gut microbiota of rats with spleen Qi deficiency syndrome
摘要
Abstract
This article aims to explore the effect and mechanism of Liujunzi Pills on the intestinal microbiota of rats with spleen Qi deficiency syndrome.The raw Rhei Radix et Rhizoma water extract(1 g·mL-1)was used to prepare spleen Qi deficiency rat models.A total of 44 SD male rats were randomly divided into a control group,a model group,Liujunzi Pills groups at high(3.24 g·kg-1),medium(1.62 g·kg-1),low(0.81 g·kg-1)doses,and Shenling Baizhu San(2.50 g·kg-1)group.The drug effect was evaluated by observing the following aspects:spleen index,fecal water content,body weight,and intestinal propulsion index.Gut microbiota analysis and 16S rRNA gene sequencing were conducted on feces.Enzyme-linked immunosorbent assay(ELISA)and UV spectrophotometry were used to detect interleukin-1β(IL-1β)and adenosine triphosphate(ATP)levels in small intestine tissues.Hematoxylin-eosin staining and transmission electron microscopy were employed to observe changes in intestinal pathology and microstructure.The results show that,compared with the control group,fecal moisture content is significantly increased while spleen index,body weight,and intestinal propulsion index are significantly reduced in rats of the model group,indicating the successful establishment of the model.The above symptoms can be improved by both Shenling Baizhu San and Liujunzi Pills.Compared with the control group,in the model group,the gut microbiota abundance is changed with an unbalanced development:the abundance of beneficial bacteria within the Bacteroidetes phylum is reduced,accompanied by a significantly decreased Shannon index,and reduced signal levels of nicotinamide adenine dinucleotide phosphate(NADPH)-related enzymes relevant to mitochondria.However,Liujunzi Pills and Shenling Baizhu San can significantly improve the Bacteroidetes phylum abundance in gut microbiota,microbial diversity,and NADPH activity in the model group.Additionally,compared with the control group,the ATP level is decreased and the IL-1β level is increased in small intestinal tissues of the model group,with shorter small intestinal epithelial villi and decreased mitochondrial number.The above symptoms can be improved by Liujunzi Pills and Shenling Baizhu San.In conclusion,Liujunzi Pills can treat spleen Qi deficiency syndrome by enhancing mitochondrial function to regulate gut microbiota balance and diversity.关键词
脾气虚证/六君子丸/肠道菌群/线粒体功能Key words
spleen Qi deficiency syndrome/Liujunzi Pills/gut microbiome/mitochondrial function引用本文复制引用
张涛,陈念,贾钦尧,雷晓霞,王杰,赵佳晴,魏莹,文静..六君子丸对脾气虚证大鼠肠道菌群的影响及机制研究[J].中国中药杂志,2025,50(15):4333-4341,9.基金项目
四川省自然科学基金一般项目(24NSFSC1730) （24NSFSC1730）
国家大学生创新创业训练计划项目(202310634002) （202310634002）
