中国中药杂志2025，Vol.50Issue(14)：3835-3846,12.DOI:10.19540/j.cnki.cjcmm.20250214.701
补阳还五汤功效成分联合使用对糖氧剥夺/复糖复氧诱导的BV2细胞炎症及氧化应激反应的影响
Effects of combined use of active ingredients in Buyang Huanwu Decoction on oxygen-glucose deprivation/reglucose-reoxygenation-induced inflammation and oxidative stress of BV2 cells
摘要
Abstract
This study aims to explore the effects and action mechanisms of the active ingredients in Buyang Huanwu Decoction(BYHWD),namely tetramethylpyrazine(TMP)and hydroxy-safflor yellow A(HSYA),on oxygen-glucose deprivation/reglucose-reoxygenation(OGD/R)-induced inflammation and oxidative stress of microglia(MG).Network pharmacology was used to screen the effective monomer ingredients of BYHWD and determine the safe concentration range for each component.Inflammation and oxidative stress models were established to further screen the best ingredient combination and optimal concentration ratio with the most effective anti-inflammatory and antioxidant effects.OGD/R BV2 cell models were constructed,and BV2 cells in the logarithmic growth phase were divided into a normal group,a model group,an HSYA group,a TMP group,and an HSYA+TMP group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of inflammatory cytokines such as interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6).Oxidative stress markers,including superoxide dismutase(SOD),nitric oxide(NO),and malondialdehyde(MDA),were also measured.Western blot was used to analyze the protein expression of both inflammation-related pathway[Toll-like receptor 4(TLR4)/nuclear factor-kappa B(NF-κB)]and oxidative stress-related pathway[nuclear factor erythroid 2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)].Immunofluorescence was used to assess the expression of proteins such as inducible nitric oxide synthase(iNOS)and arginase-1(Arg-1).The most effective ingredients for anti-inflammatory and antioxidant effects in BYHWD were TMP and HSYA.Compared to the normal group,the model group showed significantly increased levels of IL-1β,TNF-α,IL-6,NO,and MDA,along with significantly higher protein expression of NF-κB,TLR4,Nrf2,and HO-1 and significantly lower SOD levels.The differences between the two groups were statistically significant.Compared to the model group,both the HSYA group and the TMP group showed significantly reduced levels of IL-1β,TNF-α,IL-6,NO,and MDA,lower expression of NF-κB and TLR4 proteins,higher levels of SOD,and significantly increased protein expression of Nrf2 and HO-1.Additionally,the expression of the M1-type MG marker iNOS was significantly reduced,while the expression of the M2-type MG marker Arg-1 was significantly increased.The results of the HSYA group and the TMP group had statistically significant differences from those of the model group.Compared to the HSYA group and the TMP group,the HSYA+TMP group showed further significant reductions in IL-1β,TNF-α,IL-6,NO,and MDA levels,along with significant reductions in NF-κB and TLR4 protein expression,an increase in SOD levels,and elevated Nrf2 and HO-1 protein expression.Additionally,the expression of the M1-type MG marker iNOS was reduced,while the M2-type MG marker Arg-1 expression increased significantly in the HSYA+TMP group compared to the TMP or HSYA group.The differences in the results were statistically significant between the HSYA+TMP group and the TMP or HSYA group.The findings indicated that the combined use of HSYA and TMP,the active ingredients of BYHWD,can effectively inhibit OGD/R-induced inflammation and oxidative stress of MG,showing superior effects compared to the individual use of either component.关键词
补阳还五汤/糖氧剥夺/复糖复氧/炎症/氧化应激Key words
Buyang Huanwu Decoction/oxygen-glucose deprivation/reglucose-reoxygenation/inflammation/oxidative stress引用本文复制引用
夏天晴,尉杰忠,马存根,宋丽娟,陈莹,滑键林,苏琴,但存燕,戎梦玮,葛士宁,郭虹,肖保国..补阳还五汤功效成分联合使用对糖氧剥夺/复糖复氧诱导的BV2细胞炎症及氧化应激反应的影响[J].中国中药杂志,2025,50(14):3835-3846,12.基金项目
国家自然科学基金项目(82004028) （82004028）
中国博士后科学基金面上项目(2020M680912) （2020M680912）
国家中医药管理局"张仲景传承与创新专项"(GZY-KJS-2022-048-1) （GZY-KJS-2022-048-1）
山西省科技创新人才青年团队项目(202204051001028) （202204051001028）
山西中医药大学2022年度科技创新团队项目(2022TD2010) （2022TD2010）
山西省卫健委医学科技领军团队项目(2020TD05) （2020TD05）
山西中医药大学附属医院国家区域中医医疗中心心血管专项(XGZX202115) （XGZX202115）
山西中医药大学青年科学家培育项目(2021PY-QN-09) （2021PY-QN-09）
山西中医药大学学科建设经费项目(2025XKJS-02) （2025XKJS-02）
山西中医药大学2023年研究生教育创新创业项目(2023CX026) （2023CX026）
