浙江中医药大学学报2025，Vol.49Issue(8)：948-967,20.DOI:10.16466/j.issn1005-5509.2025.08.002
升降药对治疗溃疡性结肠炎作用机制比较研究
Comparative Study on the Mechanism of Action of Ascending and Descending Drugs in the Treatment of Ulcerative Colitis
摘要
Abstract
[Objective]To clarify the therapeutic effect of five groups of ascending and descending drugs on ulcerative colitis(UC),and to explore its different characteristics and potential regulatory pathways.[Methods]Sixty-four C57BL/6J male mice were randomly divided into normal control group,model control group,positive control group and five groups of ascending and descending drug groups,with 8 mice in each group.The UC model of mice was induced by dextran sulfate sodium(DSS).Except for the normal control group,the other groups of mice freely drank 2.5%DSS solution every day,and the mice in each drug group were given corresponding doses of drugs by gavage.During the modeling period,the state of the mice was observed every day,and the body weight,food intake,water intake and fecal morphology of the mice were recorded.At the end of the experiment,the disease activity index(DAI)score and colonic histopathological changes of mice in each group were compared.Transcriptome sequencing was used to analyze the differentially expressed genes in the colon of mice in each intervention group.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression level of node genes in key signaling pathway.[Results]Compared with normal control group,the DAI score of the mice in model control group was significantly increased(P<0.0001),the body weight was significantly decreased(P<0.05),the colon was significantly shortened(P<0.05),and a large number of inflammatory cell infiltration was observed.Compared with model control group,the diet of UC mice in Citri Reticulatae Pericarpium-Aurantii Fructus Immaturus group increased gradually;Platycodonis Radix-Armeniacae Semen Amarum group still had formed feces after modeling;the expression of estrogen signaling pathway-related genes closely related to pro-inflammatory response was down-regulated in Scutellariae Radix-Pinelliae Rhizoma Praeparatum Cum Zingibere Et Alumine group;the DAI index of UC mice in Atractylodis macrocephalae Rhizoma-Poria cocos group was significantly lower than that in other groups,and the liver index of UC mice in Atractylodis macrocephalae Rhizoma-Paeoniae Radix Alba group was significantly lower than that in model control group(P<0.01).The G protein-coupled receptors(GPCRs)signaling pathway was up-regulated in the Atractylodis macrocephalae Rhizoma-Poria cocos group and Atractylodis macrocephalae Rhizoma-Paeoniae Radix Alba group.[Conclusion]The five groups of ascending and descending drug pairs had different therapeutic characteristics in the treatment of UC induced by DSS.Among them,Citri Reticulatae Pericarpium-Aurantii Fructus Immaturus group,Atractylodis macrocephalae Rhizoma-Poria cocos group and Atractylodis macrocephalae Rhizoma-Paeoniae Radix Alba group had obvious improvement effects on colon tissue pathological damage and submucosal collagen deposition.The protective effect on colonic goblet cells was relatively obvious in Atractylodis macrocephalae Rhizoma-Poria cocos group and Atractylodis macrocephalae Rhizoma-Paeoniae Radix Alba group.The reduction of apolipoprotein A1(ApoA1)was most significant in Atractylodis macrocephalae Rhizoma-Poria cocos group,and the down-regulation of CXC chemokine ligand 10(CXCL10)gene expression was most obvious in Platycodonis Radix-Armeniacae Semen Amarum group.In general,the protective effect of Atractylodis macrocephalae Rhizoma-Poria cocos drug pair on UC was more comprehensive and worthy of further study.关键词
溃疡性结肠炎/药对配伍/气机失调/脏腑气机/机制研究/病理损伤/炎症反应/信号通路Key words
ulcerative colitis/drug compatibility/disordered Qi activity/Qi activity of Zang-fu viscera/mechanism research/pathological injury/inflammatory reaction/signaling pathway分类
医药卫生引用本文复制引用
杨璐榕,章语洲,李聿娜,郑迅杰,李珂,王卓晨,楼招欢,黄建波..升降药对治疗溃疡性结肠炎作用机制比较研究[J].浙江中医药大学学报,2025,49(8):948-967,20.基金项目
浙江省自然科学基金重点项目(LZ24H270002、LZ24H280001) （LZ24H270002、LZ24H280001）
国家自然科学基金项目(82374125)Zhejiang Provincial Natural Science Foundation Key Project(LZ24H270002,LZ24H280001) （82374125）
National Natural Scientific Foundation Project(82374125) （82374125）
