中国中药杂志2025，Vol.50Issue(14)：3878-3886,9.DOI:10.19540/j.cnki.cjcmm.20250416.102
基于特征图谱结合化学计量学与熵权-灰色关联度分析的栽培羌活最佳采收期研究
Optimal harvesting period of cultivated Notopterygium incisum based on HPLC specific chromatogram combined with chemometrics and entropy weight-gray correlation analysis
摘要
Abstract
To determine the optimal cultivation duration and harvest period for cultivated Notopterygium incisum and promote its industrial development,this study established a characteristic chromatographic profile of cultivated N.incisum and employed chemometrics combined with entropy-weighted grey correlation analysis to assess differences in agronomic traits and quality indicators across different cultivation years and harvest periods.By comparing with reference substances,ten common peaks were identified,including chlorogenic acid,p-coumaric acid,ferulic acid,marmesinin,nodakenin,isochlorogenic acid B,notopterol,phenethyl ferulate,isoimperatorin,and falcarindiol.The similarity between the characteristic chromatographic profiles of N.incisum at different cultivation years and the reference profile was all above 0.932.Principal component analysis(PC A)and orthogonal partial least squares discriminant analysis(OPLS-DA)revealed that the quality of 1-to 3-year-old cultivated N.incisum was highly dispersed and unstable,whereas the quality of 4-year-old cultivated N.incisum remained relatively stable across different harvest periods.This suggests that the accumulation of relevant compounds in the medicinal material had reached a plateau,confirming that the optimal cultivation period for N.incisum is four years.Entropy-weighted grey correlation analysis indicated that the quality of 4-year-old cultivated N.incisum across different harvest periods ranked from highest to lowest as follows:November,December,October,August,July,and September,demonstrating that November is the optimal harvest time.The findings of this study establish the suitable cultivation duration and optimal harvest period for N.incisum,providing a scientific basis for cultivation guidance and quality standardization.关键词
栽培羌活/特征图谱/质量评价/熵权-灰色关联度分析/采收期Key words
cultivated Notopterygium incisum/specific chromatogram/quality evaluation/entropy weight-gray correlation analysis/harvesting period引用本文复制引用
王京城,孙洪兵,刘腾,朱文涛,王红兰,周毅,王维彦,杨萍,蒋舜媛..基于特征图谱结合化学计量学与熵权-灰色关联度分析的栽培羌活最佳采收期研究[J].中国中药杂志,2025,50(14):3878-3886,9.基金项目
国家重点研发计划项目(2023YFC3503803) （2023YFC3503803）
四川省中医药管理局科技项目(2023zd029) （2023zd029）
阿坝州科技计划项目(R23TJNLJS0005) （R23TJNLJS0005）
