中国中药杂志2025，Vol.50Issue(14)：3898-3908,11.DOI:10.19540/j.cnki.cjcmm.20250308.101
不同施磷量对远志植物学性状、有效成分和根际土壤微生物群落的影响
Effect of different phosphorus application on morphological traits,active ingredients and rhizosphere soil microbial community of Polygala tenuifolia
摘要
Abstract
To investigate the effects of phosphorus fertilizer on the morphological traits,active ingredients and rhizosphere soil microbial community of Polygala tenuifolia.The phosphorus fertilizer was calculated in terms of P2O5.Five treatments were set up:0(CK),17(P1),34(P2),51(P3),and 68(P4)kg per Mu(1 Mu ≈667 m2).A randomized block design was adopted.Samples of P.tenuifolia and its rhizosphere soil were collected under different superphosphate fertilizer treatments.Illumina high-throughput sequencing was used to analyze the rhizosphere soil microbial community,9 morphological traits were measured and the content of 11 active ingredients were determined.The results showed that the whole plant weight,shoot fresh weight,root weight,and root peel thickness were the highest under Pl treatment,increasing by 34.41％,38.80％,39.21％,and 3.17％respectively compared to CK.Under P2 treatment,the plant height,stem diameter,root thickness,and core thickness were significantly higher than CK.Phosphorus fertilizer had a significant impact on the content of tenuifolin,sibiricose A5,sibiricose A6,arillanin A,3,6'-disinapoyl sucrose,and polygalaxanthone Ⅲ.Correlation analysis results showed that the relative abundance of Arthrobacter,Bacillus,norank_f_Vicinamibacteraceae,norank_o_Vicinamibacterales,MND1 and other bacteria,as well as the relative abundance of Neocosmospora,Paraphoma and other fungi were positively correlated with root diameter,wood core diameter,the whole plant weight,root weight,shoot fresh weight of P.tenuifolia.Bacillus,Neocosmospora,Subulicystidium were significantly positively correlated with oligosaccharides such as 3,6'-disinapoyl sucrose,sibiricose A5、sibiricose A6、glomeratose A、arillanin A and tenuifoliside C.Arthrobacter,Humicola,Aspergillus,Paraphoma were positively correlated with tenuifolin and norank_f_Vicinamibacteraceae,norank_o_Vicinamibacterales,Fusarium were positively correlated with polygalaxanthone Ⅲ.Evidently,appropriate phosphorus application is conducive to the growth and quality improvement of P.tenuifolia,and can increase the abundance of beneficial microorganisms in the soil.关键词
远志/磷肥/植物学性状/有效成分/土壤微生物Key words
Poly gala tenuifolia/phosphorus fertilizer/morphological traits/active ingredients/soil microorganisms引用本文复制引用
郭欢,魏通,崔文华,石欢,毛福英,谷仙,赵云生,梁晓峰..不同施磷量对远志植物学性状、有效成分和根际土壤微生物群落的影响[J].中国中药杂志,2025,50(14):3898-3908,11.基金项目
河北省自然科学基金中医药联合基金重点项目(H2O23423019) （H2O23423019）
河北省重点研发计划"中药材现代种业科技创新团队"项目(21326312D) （21326312D）
河北省现代农业产业技术体系创新团队项目(HBCT2024110205,HBCT2024110201) （HBCT2024110205,HBCT2024110201）
