中兽医医药杂志2025，Vol.44Issue(5)：29-34,6.DOI:10.13823/j.cnki.jtcvm.2025.051
加味葛根芩连汤质量控制检测方法的建立
Establishment of quality control detection method for modified Gegen Qinlian decoction
摘要
Abstract
This study aims to establish the quality control method of modified Gegen Qinlian decoction through high-performance liquid chromatography(HPLC),facilitating the modernization research and industrial production of classic prescriptions.Referring to the"Chinese Veterinary Pharmacopoeia"(Part Ⅱ)and related literature,a Hypersil ODS2 C18 column(4.6 mm×250.0 mm,5 μm)was employed.Chromatographic conditions were optimized for different components to determine the contents of puerarin,baicalin,berberine hydrochloride,liquiritin,atractylenolide,atractylon,ergosterol,and verbascoside in modified Gegen Qinlian decoction.The results indicated that the peak shapes of puerarin,baicalin,berberine hydrochloride,and liquiritin were complete,with high resolution.The negative control showed no interference at the corresponding retention times,suggesting good specificity of this chromatographic method.The RSDs of both the precision and repeatability experiments were less than 2.0%(n=6),demonstrating good precision and repeatability of the chromatographic method.The peak areas and retention times of each sample were recorded.The concentrations of puerarin,baicalin,berberine hydrochloride,and liquiritin in modified Gegen Qinlian decoction were calculated to be 0.31 mg/mL,1.52 mg/mL,0.15 mg/mL,and 0.63 mg/mL,respectively,providing a quantitative basis for the quality uniformity control of the prescription.However,atractylenolide,atractylon,ergosterol,and verbascoside were not detected in the corresponding samples,which might be attributed to the low solubility of these components or drug interactions that lead to the inability of detecting sensitively by the established method.关键词
加味葛根芩连汤/高效液相色谱/含量测定/质量控制Key words
modified Gegen Qinlian decoction/high-performance liquid chromatography/content determination/quality control分类
农业科技引用本文复制引用
王思齐,刘森,张世栋..加味葛根芩连汤质量控制检测方法的建立[J].中兽医医药杂志,2025,44(5):29-34,6.基金项目
兰州市青年科技创新项目(2023-QN-04) （2023-QN-04）
