中国中药杂志2025，Vol.50Issue(14)：3918-3929,12.DOI:10.19540/j.cnki.cjcmm.20250321.301
基于UHPLC-Q-Orbitrap HRMS技术结合指纹图谱、化学计量学比较鲜地黄"沉水"与"浮水"之异同
Comparison between sinking and floating fresh Rehmanniae Radix samples by UHPLC-Q-Orbitrap HRMS,fingerprinting,and chemometrics
摘要
Abstract
This study aims to explore the scientific connotation of sinking Rehmanniae Radix has the best quality and compare the quality between floating and sinking fresh Rehmanniae Radix samples.Ultra-performance liquid chromatography tandem quadrupole electrostatic field Orbitrap high-resolution mass spectrometry(UHPLC-Q-Orbitrap HRMS)was employed to detect the chemical components in floating and sinking fresh Rehmanniae Radix samples.The fingerprint of fresh Rehmanniae Radix was established by high performance liquid chromatography(HPLC),and four index components were determined simultaneously.The cluster analysis,principal component analysis(PCA),and orthogonal partial least squares-discriminant analysis(OPLS-DA)were conducted to compare the quality of floating and sinking fresh Rehmanniae Radix samples.An evaporative light-scattering detector was used to compare the content of five sugars.The extract yield and drying rate were determined,and the quality connotation of sinking Rehmanniae Radix has the best quality was explained by multiple indicators.A total of 41 components were preliminarily identified from fresh Rehmanniae Radix by UHPLC-Q-Orbitrap HRMS,including 7 iridoid glycosides,9 phenylethanol glycosides,6 amino acids,4 sugars,3 phenolic acids,5 nucleosides,3 organic acids,1 ionone,1 furan,1 coumarin,and 1 phenylpropanoid.The results showed that the main chemical components were consistent between floating and sinking fresh Rehmanniae Radix.Nine common peaks were identified in the fingerprints of 15 batches of floating and sinking fresh Rehmanniae Radix samples,and the similarity of fingerprints was greater than 0.9.The cluster analysis,PCA,and OPLS-DA classified floating and sinking fresh Rehmanniae Radix sasmples into two categories,indicating differences in the quality between them.The total content of catalpol,rehmannioside D,ajugol,and verbascoside in sinking fresh Rehmanniae Radix samples was higher than that in floating samples of the same batch and specification,and the main differential component was catalpol.The total content of fructose,glucose,sucrose,raffinose,and stachyose in sinking fresh Rehmanniae Radix samples was higher than that in floating samples of the same batch and specification,and the main differential component was stachyose.The extract yield and drying rate of the sinking samples were higher than those of floating samples.This study preliminarily showed that floating and sinking fresh Rehmanniae Radix samples had the same components but great differences in the content of medicinal substance basis.The total content of four glycosides and five sugars,extract yield,and drying rate of sinking fresh Rehmanniae Radix samples is higher than that of floating samples of the same batch and specification.These findings,to a certain extent,explains the scientificity of sinking Rehmanniae Radix has the best quality recorded in ancient books and provide a reference for the quality control and clinical application of fresh Rehmanniae Radix.关键词
鲜地黄/UHPLC-Q-Orbitrap HRMS/指纹图谱/含量测定/化学计量学/质量评价Key words
fresh Rehmanniae Radix/UHPLC-Q-Orbitrap HRMS/fingerprint/content determination/chemometrics/quality evaluation引用本文复制引用
刘士龙,张宏伟,张振凌,贾涵婷,郭志俊,王瑞生,张红伟,王硕,钟夷坚..基于UHPLC-Q-Orbitrap HRMS技术结合指纹图谱、化学计量学比较鲜地黄"沉水"与"浮水"之异同[J].中国中药杂志,2025,50(14):3918-3929,12.基金项目
国家重点研发计划项目(2017YFC1702800,2018YFC1707202) （2017YFC1702800,2018YFC1707202）
河南中医药大学2024年度研究生科研创新项目(2024KYCX030) （2024KYCX030）
河南省科技研发计划联合基金项目(242301420021) （242301420021）
国家药品监督管理局中药材及饮片质量控制重点实验室开放课题(KF202203) （KF202203）
