广东海洋大学学报2025，Vol.45Issue(4)：43-50,8.DOI:10.3969/j.issn.1673-9159.2025.04.005
鰤鱼诺卡氏菌弱毒株对大口黑鲈头肾转录组的影响
Effects of Attenuated Nocardia seriolae Strain on the Head Kidney Transcriptome of Largemouth Bass(Micropterus salmoides)
摘要
Abstract
[Objective]To investigate the dynamic transcriptome responses in the head kidney of largemouth bass(Micropterus salmoides)immunized with the attenuated Nocardia seriolae strain LY20810 F110,and to provide a theoretical basis for elucidating the vaccine-induced immunoprotective mechanisms.[Methods]Healthy largemouth bass were divided into immunized(injected intraperitoneally with 1×10⁸ CFU/mL attenuated N.seriolae)and control(injected with physiological saline)groups.Head kidney tissues were collected at 7 and 28 days post-immunization for RNA sequencing.Differentially expressed genes(DEGs)were identified via DESeq2 and subjected to KEGG pathway enrichment analysis.At 28 days post-immunization,fish were challenged with a N.seriolae virulent strain,and relative percent survival(RPS)was calculated alongside histopathological damage assessment.[Results]The immunized group achieved an RPS of 75%,with no granulomatous lesions or tissue damage post-challenge.Transcriptome analysis showed that 7 606 and 3 225 DEGs were identified at 7 and 28 days post-immunization,respectively.KEGG enrichment analysis revealed upregulation of innate(FcεRI signaling,complement C3)and adaptive(B-cell receptor signaling,NF-κB)immune pathways,along with increased expression of immune-related genes(IGH,CD22,PIGR),while pro-inflammatory pathways(IL-17 signaling,necroptosis)were downregulated.[Conclusion]Attenuated N.seriolae LY20810 F110 offers significant immunoprotection to largemouth bass,likely by activating innate and adaptive immune responses to eliminate pathogens and suppress excessive inflammation.关键词
鰤鱼诺卡氏菌/减毒疫苗/大口黑鲈/免疫保护/转录组Key words
Nocardia seriolae/attenuated vaccines/Micropterus salmoides/immune protection/transcriptome分类
农业科技引用本文复制引用
黄梁军,黄雷,杨娜,蔺凌云,姚嘉赟,陈静,沈锦玉,高迎莉,潘晓艺..鰤鱼诺卡氏菌弱毒株对大口黑鲈头肾转录组的影响[J].广东海洋大学学报,2025,45(4):43-50,8.基金项目
浙江省"三农九方"科技协作计划(2024SNJF051) （2024SNJF051）
湖州市公益性应用研究(2021GZ28) （2021GZ28）
浙江省"尖兵领雁+X"研发攻关计划(2024C02005) （2024C02005）
浙江省科研院所专项(2025YSZX02) （2025YSZX02）
