中药药理与临床2025，Vol.41Issue(8)：28-33,6.
小草丸对寒凝血瘀心肌缺血大鼠血液流变学及心脏损伤的影响
Effect of Xiaocao(小草)Pills on Hemorheology and Heart Injury in Rats with Myocardial Ischemia of Hanningxueyu Syndrome
摘要
Abstract
Objective:To study the effect of Xiaocao(小草)Pills on hemorheology and heart injury in rats with myocardial ischemia of Hanningxu-eyu(寒凝血瘀)syndrome.Methods:Rats were randomly divided into normal control group,model control group,10 mg/kg aspirin group,85 mg/kg compound Danshen Dripping Pill group,and 0.5,1.5,and 4.5 g/kg Xiaocao Pill groups.Rats were administrated with correspond-ing drugs.After 1 h of administration,except for the normal control group,rats in other groups were placed in 0-4℃cold stimulation for 5 min,and isoproterenol(5 mg/kg)was injected intraperitoneally,for seven days continuously.The body mass of rats in each group was measured at 12 h and 24 h after the last administration.The cardiac ultrasonic function,hemorheology level,thrombin level,creatine kinase isoenzyme-MB(CK-MB)activity,cardiac troponin-T(CTn-T),von Willebrand factor(VWF),platelet α-granule membrane protein 140(GMP-140),cyclic adenosine monophosphate(cAMP),and cyclic guanosine monophosphate(cGMP)were measured.Hematoxylin-Eosin(HE)stai-ning was used to observe the pathological changes in the cardiac muscle tissue of rats and calculate the score.Results:Compared with that in the normal control group,the rats'body mass(on the 3rd,5th,and 7th day)was reduced in the model control group,as well as the contents of cAMP and cGMP(P<0.05,P<0.01).The left ventricular ejection fraction(LVEF),left ventricular fractional shortening(LVFS),stroke vol-ume(SV),cardiac output(CO),hemorheological indexes,prothrombin time(PT),activated partial thromboplastin time(APTT),CK-MB,CTN-T,cardiac mass index,VWF,GMP-140,and fibrinogen(FIB)were increased(P<0.05,P<0.01).Compared with the model control group,Xiaocao Pills could significantly increase the body mass of rats(on the 3rd,5th,and 7th day)and the contents of cAMP and cGMP(P<0.05,P<0.01).The levels of LVEF,LVFS,SV,CO,hemorheological indexes,PT,APTT,CK-MB,CTN-T,cardiac mass index,VWF,GMP-140,and FIB were decreased(P<0.05,P<0.01).HE staining showed that the myocardial injury,inflammatory infiltration,and myo-cardial cell widening in Xiaocao Pill groups were obviously improved,and the pathological score was significantly reduced.Conclusion:Xiao-cao Pills can effectively regulate the cardiac function,hemorheology,and coagulation indexes of rats with myocardial ischemia of Hanningxueyu syndrome and play a role in improving blood viscosity and myocardial injury,and its mechanism may be related to regulating the levels of VWF,GMP-140,cAMP,and cGMP.关键词
小草丸/寒凝血瘀心肌缺血模型/血液流变学/心肌损伤/心肌肌钙蛋白-T/血管性血友病因子/血小板α-颗粒膜蛋白Key words
Xiaocao(小草)Pills/Myocardial Ischemia Model of Hanningxueyu(寒凝血瘀)Syndrome/Hemorheology/Myocardial Injury/CTn-T/VWF/GMP-140引用本文复制引用
张欣蕾,王文文,杨雯,栗学超,朱小红,阎博,张红,房敏峰..小草丸对寒凝血瘀心肌缺血大鼠血液流变学及心脏损伤的影响[J].中药药理与临床,2025,41(8):28-33,6.基金项目
陕西省重点研发计划(编号:2018ZDXM-SF-014) （编号:2018ZDXM-SF-014）
"秦药"研发重点实验室(编号:2021-QYPT-001) （编号:2021-QYPT-001）
"秦药"质量提升工程项目(编号:2021-QYZL-01) （编号:2021-QYZL-01）
陕西省教育厅服务地方专项(编号:2018JC032) （编号:2018JC032）
陕西省药品监督管理局药品科学监管和监管科学研究项目(编号:SXYJ202104). （编号:SXYJ202104）
