中药药理与临床2025，Vol.41Issue(8)：40-44,39,6.
黄芪、莪术配伍抑制肿瘤相关成纤维细胞介导的肝癌转移的机制研究
Mechanism of Huangqi(黄芪)-Ezhu(莪术)in Inhibiting Hepatocellular Carcinoma Metastasis Mediated by Cancer-Associated Fibroblasts
摘要
Abstract
Objective:To observe the effect and mechanism of Huangqi(黄芪)-Ezhu(莪术)on hepatocellular carcinoma(HCC)metastasis media-ted by cancer-associated fibroblasts(CAFs).Methods:The co-culture system of conditioned medium was established.Cell proliferation and migration were detected by the cell-counting kit 8(CCK8)method and Transwell assay,respectively.The level of C-C motif chemokine ligand 5(CCL5)secreted by CAFs was measured by enzyme-linked immunosorbent assay(ELISA).The mRNA levels of matrix metalloproteinase(Mmp)2 and Mmp9 were determined by real-time PCR.In addition,the protein levels of MMP 2,MMP 9,C-C motif chemokine receptor 5(CCR5),phospho-mitogen-activated extracellular signal-regulated kinase(p-MEK),and phospho-extracellular signal-regulated kinase(p-ERK)were determined by Western blotting.Huh7 cells and CAFs with overexpression of CCL5 were injected into the yolk sac area of ze-brafish,respectively,to construct the zebrafish model of HCC metastasis.The number of Huh7 cells migrating in the tail was counted by laser confocal microscopy.Results:Huangqi-Ezhu inhibited the Huh7 cell migration mediated by CAFs(P<0.01),lowered the level of CCL5 se-creted by CAFs(P<0.01),and down-regulated the mRNA and protein levels of Mmp2 and Mmp9 as well as the protein levels of CCR5,p-MEK,and p-ERK in Huh7 cells mediated by CAFs(P<0.05 or P<0.01).Moreover,Huangqi-Ezhu inhibited the Huh7 cell migration media-ted by CAFs with overexpression of CCL5 in zebrafish(P<0.01).Conclusion:Huangqi-Ezhu can inhibit Huh7 cell metastasis mediated by CAFs by inhibiting the CCL5/CCR5/MEK/ERK signaling pathway.关键词
黄芪、莪术/配伍/肿瘤相关成纤维细胞/肝细胞肝癌/转移/趋化因子配体5Key words
Hunagqi(黄芪)/Ezhu(莪术)/Compatibility/Cancer-associated fibroblasts/Hepatocellular carcinoma/Metastasis/C-C motif chemokine ligand 5引用本文复制引用
张硕,唐德才,姜晓燕,杨水英,谭喜莹..黄芪、莪术配伍抑制肿瘤相关成纤维细胞介导的肝癌转移的机制研究[J].中药药理与临床,2025,41(8):40-44,39,6.基金项目
国家自然科学基金项目(编号:82104408、82003961). （编号:82104408、82003961）
