中华中医药学刊2025，Vol.43Issue(9)：192-196,后插38,6.DOI:10.13193/j.issn.1673-7717.2025.09.035
肉苁蓉调控Keap1/Nrf2/HO-1抗氧化通路改善帕金森病模型大鼠海马神经细胞损伤
Roucongrong(Cistanches Herba)Ameliorates Nerve Cell Injury of Hippocampus in Parkinson's Disease Rats through Regulating Keap1/Nrf2/HO-1 Antioxidant Pathway
摘要
Abstract
Objective To explore the effect of Roucongrong(Cistanches Herba)on nerve cell injury in Parkinson's disease(PD)rats and its related mechanism.Methods Sprague-dawley(SD)male rats were randomly divided into three groups(10 rats in each group):control group,model group and Roucongrong(Cistanches Herba)group.The rats in the model group and the Roucongrong(Cistanches Herba)group were intracranially injected with 6-hydroxydopamine(6-OHDA).The rats in the Rou-congrong(Cistanches Herba)group were given Roucongrong(Cistanches Herba)solution(0.216 g/mL),and those in the blank group and model group were given the normal saline irrigation[1 mL(100 g·d)]for two weeks.The morphology and ultrastruc-ture of nerve cells in hippocampus were observed by hematoxylin-eosin staining and transmission electron microscopy.The mor-phology of Nishi bodies in the Hemimani region was observed by Nishi's staining.The levels of malondialdehyde(MDA)and su-peroxide dismutase(SOD)were detected by thiobarbituric acid method and nitroblue tetrazolium method.The changes of Kelch-like ECH-related protein 1(Keap 1),nuclear factor E2-related factor 2(Nrf 2)and heme cyclooxygenase 1(HO-1)proteins were analyzed by Western blot.Results After the treatment of Roucongrong(Cistanches Herba),the hippocampal tissue structure of PD rats was improved,the morphology of neurons was improved,the nuclear pyretosis was relieved,the content of antioxidant enzyme SOD in hippocampus was gradually increased,while the content of oxidative stress product MDA was gradually decreased,and the expression of Keap 1 protein in hippocampus was decreased.The protein expressions of Nrf 2 and HO-1 increased.Conclusions Roucongrong(Cistanches Herba)can improve the damage of hippocampus neurons,and reduce the oxidative stress response in hippocampus of PD rats.The effect of Roucongrong(Cistanches Herba)in the treatment of PD rats is closely related to the regulation of Keap-1/Nrf 2/HO-1 signaling pathway.关键词
肉苁蓉/帕金森病/氧化应激/海马/神经细胞/Keap1/Nrf2/HO-1Key words
Roucongrong(Cistanches Herba)/Parkinson's disease/oxidative stress/hippocampus/neurons/Keap1/Nrf2/HO-1分类
医药卫生引用本文复制引用
许茜,林瑶,高正涛,范自立,全鑫,林萍燕,周炳灿,刘海鑫..肉苁蓉调控Keap1/Nrf2/HO-1抗氧化通路改善帕金森病模型大鼠海马神经细胞损伤[J].中华中医药学刊,2025,43(9):192-196,后插38,6.基金项目
福建省中医药科研计划项目(2021zyjc02) （2021zyjc02）
福建中医药大学中医健康管理学联合省级临床重点专科建设项目(XJG2023018) （XJG2023018）
