作物研究2025，Vol.39Issue(4)：351-360,10.
水稻籼型不育系荣丰A稻瘟病抗性的分子标记辅助改良
Molecular Marker-Assisted Improvement of Blast Resistance in Indica CMS Line Rongfeng A
摘要
Abstract
To enhance the blast resistance of the indica cytoplasmic male-sterile line Rongfeng A,this study utilized rice cultivar Gumei No.4,which carries the broad-spectrum and durable blast resistance gene Pigm,as the donor parent to con-duct marker-assisted selection(MAS)on the indica cytoplasmic male-sterile line Rongfeng A and its corresponding main-tainer line Rongfeng B,employing the codominant functional marker M143104 associated with Pigm.Field trials were con-ducted to evaluate blast resistance,sterility characteristics,agronomic traits,and yield-related traits,aiming to select the optimal improved sterile line and its corresponding maintainer line with superior comprehensive traits.The results showed that Rongfeng A-Pigm-3 exhibited high resistance to blast and complete sterility,with a sterility rate and sterility degree both at 100%.The total stigma exsertion rate was 54.3%,the panicle enclosure rate was 21.8%,the growth duration from sowing to heading was 65 days,the plant height was 52.6 cm,the effective panicles per plant were 20.3,the main panicle length was 21.5 cm,and the number of spikelets per main panicle was 124.1.The maintainer line Rongfeng B-Pigm-3 al-so demonstrated high blast resistance,with a growth duration of 63 days,plant height of 71.1 cm,effective panicles per plant of 17.9,main panicle length of 21.3 cm,spikelets per main panicle of 131.5,seed setting rate of 74.8%,1 000-grain weight of 23.5 g,and theoretical yield per plant of 41.4 g.Ultimately,this study successfully selected the superior three-line sterile line Rongfeng A-Pigm-3 and its maintainer line Rongfeng B-Pigm-3,which exhibits excellent sterility characteristics and outstanding comprehensive agronomic traits,providing high-quality germplasm resources for blast resis-tance breeding in rice.关键词
水稻/稻瘟病/抗性改良/分子标记辅助选择/荣丰AKey words
rice/rice blast resistance improvement/molecular marker-assisted selection/Rongfeng A分类
农业科技引用本文复制引用
谌强,黄俊,邹玉莹,邓吉奇,李家鑫,郭成龙,刘雄伦,刘金灵..水稻籼型不育系荣丰A稻瘟病抗性的分子标记辅助改良[J].作物研究,2025,39(4):351-360,10.基金项目
国家自然科学基金(31972256) （31972256）
湖南省科技计划重大专项(2021NK1001). （2021NK1001）
