作物学报2025，Vol.51Issue(10)：2570-2580,11.DOI:10.3724/SP.J.1006.2025.54038
棉花减数分裂进程鉴定体系构建及其对高温胁迫的响应分析
Construction of a meiotic progression identification system in cotton and analysis of its response to high-temperature stress
摘要
Abstract
High-temperature stress has become a critical factor disrupting cotton meiosis and reducing yield.In this study,using the cotton transformation receptor material'Jin 668',we systematically established a reliable method for identifying meiotic pro-gression in cotton by optimizing protocols for hydrochloric acid hydrolysis,enzymatic digestion,and acetic acid maceration of cotton flower buds.This optimized system effectively minimized interference from impurities during slide preparation.By exam-ining the morphology of microspore mother cells and chromosomes in buds of varying lengths,we clarified the relationship be-tween bud length and meiotic stage,and characterized cellular and chromosomal features across key stages,including prophase I,metaphase I,anaphase I,telophase I,metaphase II,anaphase II,and telophase II.Our results indicate that high-temperature stress significantly affects the early stages of prophase I,leading to abnormal chromosome condensation,failure of homologous pairing,disrupted crossover recombination,and ultimately defective microspore development.The identification system we developed provides a robust technical foundation for further investigation into cotton meiosis and its response to high-temperature stress,offering valuable strategies for breeding heat-tolerant cotton varieties and facilitating genetic improvement efforts.关键词
棉花/高温胁迫/减数分裂/染色体行为/制片技术Key words
cotton/high-temperature stress/meiosis/chromosome behavior/slide preparation technique引用本文复制引用
李亚玮,徐盈盈,左春阳,刘若男,梁亚军,孔杰,张献龙,闵玲..棉花减数分裂进程鉴定体系构建及其对高温胁迫的响应分析[J].作物学报,2025,51(10):2570-2580,11.基金项目
本研究由新疆维吾尔自治区 2023 年"揭榜挂帅"棉花品种重大农艺性状解析与分子设计育种项目(2023A01-3),新疆人才发展基金"优质耐高温棉花关键基因发掘与育种应用"项目(202403-03)和湖北省杰出青年基金-棉花响应高温胁迫遗传机制解析及育种应用项目(2024AFA059)资助. This study was supported by the"Unveiling and Commanding"Major Agronomic Traits and Molecular Design Breeding in Cotton Varieties of Xinjiang Uyghur Autonomous Region(2023A01-3),the Projects Sponsored by the Development Fund for Xinjiang Talents"Exploration and Breeding Application of Key Genes for High-Quality,Heat-Resistant Cotton"(202403-03),and the Hubei Provincial Outstanding Youth Fund-Genetic Mechanism Analysis and Breeding Application of Cotton in Response to High Temperature Stress(2024AFA059). （2023A01-3）
