作物学报2025，Vol.51Issue(10)：2595-2604,10.DOI:10.3724/SP.J.1006.2025.53023
ZmMS13不同突变产生了gms1和yems1166复等位玉米雄性不育系
Different mutations in ZmMS13 generate the gms1 and yems1166 multiple allele maize male-sterile lines
摘要
Abstract
The development and utilization of maize male-sterile lines is an effective strategy to reduce the cost of hybrid seed production and improve seed purity.In this study,two maize male-sterile mutants,gms1 and yems1166,were identified.Pheno-typic analysis revealed that both mutants exhibited abnormal anther cuticle structures,microspore degradation at the late uninu-cleate stage,and complete absence of mature pollen grains.Genetic analysis indicated that male sterility in both mutants is con-trolled by a single recessive nuclear gene.Using a positional cloning approach,the GMS1 and YEMS1166 loci were mapped to intervals of 23.52-26.09 Mb and 24.86-30.95 Mb on chromosome 5,respectively,regions containing the previously reported male fertility gene ZmMS13,which encodes the ABCG transporter ZmABCG2a.Gene sequencing revealed that gms1 carries a single base substitution(TCA>TGA)in the fifth exon of ZmMS13,resulting in a premature stop codon.The yems1166 mutant harbors an 8-bp deletion in the first exon of the same gene,also leading to a premature stop codon.Allelic tests confirmed that gms1 and yems1166 are novel allelic variants of ZmMS13.These findings identify two new allelic mutants of ZmMS13 and pro-vide valuable germplasm resources for the development of male-sterile lines in maize hybrid seed production.关键词
玉米/雄性不育/角质层/ms13/ABCG转运蛋白Key words
maize/male sterility/anther cuticle/ms13/ABCG transporter引用本文复制引用
赵志文,陈晓阳,陈慧,连玉杰,陆涵,曹旭东,王帆,喻梦璠,张战辉,汤继华..ZmMS13不同突变产生了gms1和yems1166复等位玉米雄性不育系[J].作物学报,2025,51(10):2595-2604,10.基金项目
本研究由河南省科技研发计划联合基金(优势学科培育类)项目(242301420131)和郑州市"揭榜挂帅"制重点研发专项(2023JBGS013)资助. This study was supported by the Joint Foundation for Science and Technology Research and Development Program of Henan Province(Cul-tivation of Superior Disciplines)(242301420131)and the Zhengzhou Research and Development Project of Open Bidding for Selecting the Best Candidates(2023JBGS013). （优势学科培育类）
