作物学报2025，Vol.51Issue(10)：2605-2618,14.DOI:10.3724/SP.J.1006.2025.53014
一种玉米穗腐病新病原棘孢木霉的分离与鉴定
Isolation and identification of a new pathogen Trichoderma asperellum causing ear rot in maize
摘要
Abstract
Maize ear rot,caused by pathogenic fungi,significantly reduces both yield and grain quality.More than 20 pathogens have been reported to cause this disease,with F.verticillioides and F.graminearum being the most prevalent in China.In recent years,a distinct form of ear and grain rot,characterized by white hyphae and greenish-blue spores,has been frequently observed in various maize-growing regions across the country.However,the specific pathogen responsible for this type of ear rot had not been clearly identified.In this study,we identified the pathogen using a combination of morphological characterization,rDNA-ITS sequencing,and whole-genome sequencing.The results revealed that T.asperellum is the causative agent of maize greenish ear rot.A representative strain,Tr.10,was selected for pathogenicity re-evaluation by inoculating 419 maize inbred lines with a conidial suspension using ear needle-inoculation.The results confirmed that Tr.10 conidia successfully reinfected a wide range of inbred lines,inducing typical T.asperellum ear rot symptoms.Significant variation in disease resistance was observed across the tested lines.Among the 419 genotypes,6.8%were classified as highly resistant,30.4%as resistant,30.7%as moder-ately resistant,24.9%as susceptible,and 7.1%as highly susceptible.Notably,25 inbred lines,including PHN47,F321,and Jing 2416K,exhibited high levels of resistance.These findings provide important insights into resistance mechanisms and offer a foundation for molecular breeding of Trichoderma ear rot-resistant maize cultivars.关键词
玉米穗腐病/棘孢木霉/致病性/果穗针刺接种法/抗性鉴定Key words
maize ear rot/T.asperellum/pathogenesis/needle spike cob inoculation/resistance identification引用本文复制引用
肖森林,王荣焕,宋伟,王维香,赵久然,阙凡,周治寰,张海霞,邢锦丰,朱祥彰,张彦冰,张楠,孙轩..一种玉米穗腐病新病原棘孢木霉的分离与鉴定[J].作物学报,2025,51(10):2605-2618,14.基金项目
本研究由北京市农林科学院科技创新能力建设专项(KJCX20240332)和国家自然科学基金项目(31871638)资助. This study was supported by the Special Project for Science and Technology Innovation Capacity Building of Beijing Academy of Agricul-tural and Forestry Sciences(KJCX20240332)and the National Natural Science Foundation of China(31871638). （KJCX20240332）
