中药药理与临床2025，Vol.41Issue(7)：32-37,6.
敦煌神仙粥调控GSK-3β/Nrf2信号通路缓解放射性脑损伤
Dunhuang Shenxian(敦煌神仙)Porridge Alleviates Radiation-Induced Brain Injury by Regulating GSK-3β/Nrf2 Signaling Pathway
摘要
Abstract
Objective:To study the protective effect of Dunhuang Shenxian(敦煌神仙)porridge on radiation-induced brain injury and its relation-ship with oxidative stress and glycogen synthase kinase-3β(GSK-3β)/nuclear factor erythroid 2-related factor 2(Nrf2)signaling pathway.Methods:Forty SD rats were randomly divided into five groups:the normal control,the model control,and Shenxian porridge(4.8,9.6,and 19.2 g/kg),with 8 rats in each group.After 7 days of routine feeding,the brains of rats in other groups except the normal control group were irradiated with a single dose of 20 Gy X-ray.After irradiation,the rats in Shenxian porridge groups were administered with Shenxian porridge at corresponding doses by gavage,and those in other groups were administered with the same amount of saline by gavage for 14 days.After the intervention,the cognitive function of rats was examined by the Morris water maze test,and the protein levels of GSK-3β,phosphorylated GSK-3β(p-GSK-3β),and Nrf2 in the brain tissues were determined by Western blot.Reactive oxygen species(ROS)in the brain tissue was de-tected by immunofluorescence.The levels of malondialdehyde(MDA)and glutathione(GSH)in the brain tissues were measured by colorime-try.The levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in the serum were determined by ELISA.Hematoxylin-eosin staining was employed to observe the histomorphological changes of the rat brain.Results:Compared with the normal control group,the rats in the model control group showed prolonged escape latency and space exploration time,elevated levels of TNF-α,IL-1β,ROS,and MDA,up-reg-ulated expression of p-GSK-3β,a lowered level of GSH,down-regulated expression of Nrf2,and a decreased body weight(P<0.01).Com-pared with the model control group,the rats in Shenxian porridge(9.6 g/kg)group showed shortened escape latency and space exploration time,lowered levels of TNF-α,IL-1β,ROS,and MDA at different time points after radiation,down-regulated expression of p-GSK-3β,an ele-vated level of GSH,up-regulated expression of Nrf2,and an increased body weight(P<0.05 or P<0.01).Conclusion:Shenxian porridge has a protective effect on the brain injury induced by X-ray.It can down-regulate the expression of p-GSK-3β,lower the levels of TNF-α and IL-1β,and up-regulate the expression of Nrf2,thereby regulating oxidative stress and inflammation to alleviate the radiation-induced injury in the brain tissue.关键词
神仙粥/辐射/脑损伤/氧化应激/保护/磷酸化糖原合成酶激酶-3β/核因子E2相关因子2Key words
Shenxian(神仙)porridge/Radiation/Brain injury/Oxidative stress/Protection/p-GSK-3β/Nrf2引用本文复制引用
刘秀珠,马礼科,马玉,武兵兵,王燕如,李佳蔚,何进鹏,谢小冬,刘永琦..敦煌神仙粥调控GSK-3β/Nrf2信号通路缓解放射性脑损伤[J].中药药理与临床,2025,41(7):32-37,6.基金项目
高校科研创新平台重大培育项目(编号:2024CXPT-08) （编号:2024CXPT-08）
甘肃中医药大学研究生创新基金资助项目(2021CX27) （2021CX27）
2023年甘肃省海智基地特色示范项目"健康管理基因检测产品的研发"(编号:GSHZTS 2022-04) （编号:GSHZTS 2022-04）
甘肃省科技重大专项(编号:22ZD1FA001). （编号:22ZD1FA001）
