中药药理与临床2025，Vol.41Issue(7)：52-56,5.
微生物转化补骨脂及其成分抗CKD血管钙化活性研究
Microbial Biotransformation of Psoralea corylifolia and Its Components for Targeting Vascular Calcification in Chronic Kidney Disease(CKD)
摘要
Abstract
Objective:To investigate the potential of Psoralea corylifolia in the prevention and treatment of chronic kidney disease(CKD)and evalu-ate the anti-vascular calcification activity of its components after microbial biotransformation.Methods:The optimal microbial strain for the fermentation of Psoralea corylifolia was screened.A CKD vascular calcification mouse model was established by using a high-purine diet.The biotransformed Psoralea corylifolia was administered to intervene in model mice.Experimental mice were randomly divided into a normal con-trol group,a model group,a fermented Psoralea corylifolia group,and an unfermented Psoralea corylifolia group.Gavage intervention was con-ducted for 60 consecutive days.Serum markers of renal function were measured,and the kidney tissue structure was analyzed by using hema-toxylin-eosin(HE)and Masson's staining.Vascular calcification was assessed through Von Kossa and alizarin red staining.Results:The strain screening resulted in Saccharomyces cerevisiae as the best strain.After intervention by Psoralea corylifolia and unfermented Psoralea corylifolia,the decreasing degree of serum renal function indexes of CKD vascular calcification mice was as follows:the normal control group<the fermented Psoralea corylifolia group<the unfermented Psoralea corylifolia group<the model group.HE staining revealed that fermented Psoralea corylifolia significantly reduced adenine crystal deposition in kidney tissue and alleviated glomerular and tubular damage.Masson's staining indicated that fermented Psoralea corylifolia effectively mitigated renal fibrosis.Von Kossa and alizarin red staining demonstrated that fermented Psoralea corylifolia markedly suppressed aortic calcification and calcium salt deposition in CKD vascular calcification mice.Conclusion:The transformation of Psoralea corylifolia by Saccharomyces cerevisiae significantly inhibited renal fibrosis and vascular calcifica-tion in CKD mouse models.These findings suggest that microbial transformation enhances the therapeutic efficacy of Psoralea corylifolia a-gainst CKD-associated vascular calcification.关键词
补骨脂/微生物发酵/慢性肾脏病/血管钙化Key words
Psoralea corylifolia/Microbial fermentation/Chronic kidney disease/Vascular calcification引用本文复制引用
石梦琴,肖文艳,罗光应,马卫悦,姚瑞娇,蒋文艺,何正有,任思冲..微生物转化补骨脂及其成分抗CKD血管钙化活性研究[J].中药药理与临床,2025,41(7):52-56,5.基金项目
四川省科技厅面上项目(编号:2024NSFSC0687) （编号:2024NSFSC0687）
四川省中医药管理局面上项目(编号:2023MS203) （编号:2023MS203）
成都医学院第一附属医院重点项目(编号:CYFY2021ZD01) （编号:CYFY2021ZD01）
成都医学院自然科学重点项目(编号:CYZZDY22-01) （编号:CYZZDY22-01）
四川省抗感染药物创制工程研究中心开放课题(编号:KGR202402、KGR202403). （编号:KGR202402、KGR202403）
