大麦与谷类科学2025，Vol.42Issue(4)：1-9,9.DOI:10.14069/j.cnki.32-1769/s.2025.04.001
黄淮稻区水稻抽穗期基因Hd1多态性分析及关键位点分子标记开发
Polymorphic Analysis of Heading Date Gene Hd1 of Rice Varieties in Huang-Huai Region and Development of Molecular Markers of Key Genotypes
摘要
Abstract
Heading date is one of the most important agronomic traits in rice.In order to clarify the polymorphism of Hd1 genotype in rice varieties in Huang-Huai rice region and the regulatory effect of the different genotypes on heading date,this study compared the sequences of Hd1 gene of 54 rice varieties in Huang-Huai rice region and analyzed the heading date and maturity date of rice with different Hd1 genotypes.The results showed that 12 different mutant sites of Ginbouzu genotype in the tested varieties were detected,and were classified as 8 kinds of Type A-H genotypes.Type A genotype was detected in 3 geng rice varieties.Type B genotype was detected in 10 geng rice varieties.Type C genotype was detected in 2 geng rice introduced from Japan.Type D genotype was detected in 3 geng rice varieties.Type E was detected in only 1 geng rice variety.Type F,Type G and Type H were only detected in indica rice varieties.The Ginbouzu Hd1 genotype was detected in 28 geng rice varieties.The maturity date of rice varieties with Type A was significantly advanced,but the heading date was not significantly advanced.The heading date and maturity date of rice varieties with Type B were significantly delayed.Both Type C and Type D genotypes had a significant advance effect on heading and maturity of rice.The molecular marker of Type D genotype was developed and verified.The length of PCR product of Type D genotype was 107 bp,and that of Ginbouzu genotype was 150 bp,which could effectively distinguish the two different functional genotypes.关键词
抽穗期/Hd1/等位基因/调控效应/分子标记Key words
Heading date/Hd1/Alleles/Regulating effect/Molecular marker分类
农业科技引用本文复制引用
李凡,陈春,郭新亚,王飞飞,陈卫军..黄淮稻区水稻抽穗期基因Hd1多态性分析及关键位点分子标记开发[J].大麦与谷类科学,2025,42(4):1-9,9.基金项目
宿迁市科技计划(K202243). （K202243）
