法医学杂志2025，Vol.41Issue(3)：201-207,7.DOI:10.12116/j.issn.1004-5619.2024.441004
NETosis标记物CitH3在小鼠深静脉血栓中的时序性表达
Temporal Expression of NETosis Marker CitH3 in Deep Vein Thrombosis in Mice
摘要
Abstract
Objective To detect the expression changes of citrullinated histone H3(CitH3)during the development of deep vein thrombosis(DVT)in mice,and to explore its value in estimating the time to thrombosis.Methods The inferior vena cava(IVC)of mice was ligated to establish a thrombosis model induced by congestion.Mice were sacrificed under excessive anesthesia at 0 h,1 d,3 d,5 d,7 d,10 d,14 d and 21 d after the modeling,respectively.The congested IVC segments(0 h after modeling)and the thrombosed IVC segments(1-21 days after modeling)were extracted.Immunohistochemistry and double immunofluorescence staining were used to observe the number of neutrophils and the ex-pression of CitH3 during thrombosis.Western blotting was used to detect the protein expression level of CitH3.Results During thrombosis,CitH3 was mainly expressed in neutrophils within the thrombus.A small number of neutrophils and a few CitH3-positive cells were observed at 0 h after modeling in the congested IVC.Between 1 d and 21 d after modeling,the number of neutrophils reached a peak at 1 d and gradually decreased.The number of CitH3-positive cells and their ratio to neutrophils began to increase at 1 d,reached a peak at 5 d after modeling,and then decreased.The expression level of CitH3 protein began to increase at 1 d and reached a peak at 5 d after modeling.Conclusion The expres-sion of CitH3 during DVI shows temporal changes,and is expected to become a biological marker for estimating the formation time of thrombosis.关键词
法医病理学/深静脉血栓/瓜氨酸化组蛋白H3/时序性表达/小鼠Key words
forensic pathology/deep vein thrombosis/citrullinated histone H3(CitH3)/temporal expres-sion/mice分类
医药卫生引用本文复制引用
王黔,杨嵩民,吴娟娟,张煜,王祥孟,陈刚,蒋朋飞..NETosis标记物CitH3在小鼠深静脉血栓中的时序性表达[J].法医学杂志,2025,41(3):201-207,7.基金项目
浙江省自然科学基金资助项目(LY20H150006) （LY20H150006）
