法医学杂志2025，Vol.41Issue(3)：252-259,8.DOI:10.12116/j.issn.1004-5619.2023.531101
国产人类DNA定量试剂盒的验证及法医学应用
Validation and Forensic Application of a Domestic Human DNA Quantitative De-tection Kit
摘要
Abstract
Objective To verify the efficacy of a domestic human DNA quantification kit based on real-time fluorescence quantitative PCR in detecting the total human DNA concentration,male DNA concen-tration in mixed male/female DNA samples,the degree of DNA degradation and inhibitor tolerance.Methods Samples with different concentrations,different male/female ratios,different concentrations of inhibitors,and different degradation degrees were tested using the domestic human DNA quantification kit based on real-time fluorescence quantitative PCR.This kit was compared with a similar product on the market and was applied to the detection of DNA from real cases.Results This human DNA quan-tification kit can effectively detect human DNA as low as 0.001 65 ng/μL,and 6.25 pg/μL of male DNA in mixed samples with a male-to-female ratio of 1∶15 000.Even when the sample contains as high as 400 ng/μL of humic acid or 1 000 μmol/L of hemin alone,the DNA concentration can still be accurately detected.The degradation index can effectively characterize the degradation degree of the sample.This kit has been successfully applied in forensic practice.Conclusion This human DNA quan-tification kit is accurate and reliable in detection.It can accurately reflect the degradation of DNA and inhibitor tolerance.It has good performance in quantitative accuracy,determination of the male/female ratio in mixed samples,and inhibitor tolerance.It has application potential in forensic case examination.关键词
法医遗传学/DNA定量/短串联重复序列/实时定量PCR/检测效能Key words
forensic genetics/DNA quantification/short tandem repeat(STR)/real-time quantitative PCR/detection effectiveness分类
医药卫生引用本文复制引用
陈静,刘志芳,陈健刚,王亚萍,冯云鹏,胡晓欣,贾振军,刘洪迪,严安心,李永久,彭柱..国产人类DNA定量试剂盒的验证及法医学应用[J].法医学杂志,2025,41(3):252-259,8.基金项目
中央级公益性科研院所基本科研业务费专项(2022JB019) （2022JB019）
