分析化学2025，Vol.53Issue(8)：1213-1225,13.DOI:10.19756/j.issn.0253-3820.251144
双响应三磷酸腺苷荧光探针用于生物成像的研究进展
Advances in Dual-response Adenosine Triphosphate Fluorescent Probes for Bioimaging
摘要
Abstract
Adenosine triphosphate(ATP),as the core energy metabolism molecule in living systems,has dynamic changes closely related to fundamental physiological processes.To meet the urgent demand for spatiotemporal ATP detection in vivo and in situ,the development of highly sensitive multifunctional synchronous sensing fluorescent probes has become a recent research focus.These dual-function probes achieve fluorescence detection of dual targets by designing recognition sites for ATP alongside biological factors or microenvironment parameters such as reactive oxygen/nitrogen/sulfur species,metal ions,and enzymes,enabling physiological/pathological state correlation analysis through bioimaging.This paper systematically reviews recent advances in fluorescent probes for the collaborative detection of ATP and key biomolecules.It specifically examines probe construction strategies based on specific molecular recognition mechanisms(e.g.,metal coordination competition,electrostatic interactions,and host-guest recognition),multi-modal optical signal transduction mechanisms(ratiometric fluorescence,fluorescence lifetime,and photodynamic therapy),and their applications in pathological models such as oxidative stress monitoring,metal homeostasis imbalance,and enzyme activity co-detection.Finally,from the perspective of molecular probe engineering,current challenges and future research directions are proposed to provide methodological support for precise analysis of ATP-related life process regulation networks.关键词
多功能探针/荧光传感/三磷酸腺苷/生物活性物质/生物成像/评述Key words
Multifunctional probes/Fluorescence sensing/Adenosine triphosphate/Bioactive substances/Bioimaging/Review引用本文复制引用
徐庆雨,李想,曹伟,彭志华,曾景斌..双响应三磷酸腺苷荧光探针用于生物成像的研究进展[J].分析化学,2025,53(8):1213-1225,13.基金项目
国家自然科学基金项目(No.22476214)、山东省自然科学基金杰出青年基金项目(No.ZR2022JQ07)、泰山学者青年专家项目(No.tsqn202211080)、中央高校基本科研业务费项目(No.24CX03011A)和青岛市自然科学基金重点项目(No.24-8-4-zrjj-9-jch)资助. Supported by the National Natural Science Foundation of China(No.22476214),the Key Fundamental Project of Shandong Natural Science Foundation(No.ZR2022JQ07),the Taishan Scholarship of Shandong Province(No.tsqn202211080),the Fundamental Research Funds for the Central Universities(No.24CX03011A)and the Key Fundamental Project of Qingdao City(No.24-8-4-zrjj-9-jch). （No.22476214）
