分析化学2025，Vol.53Issue(8)：1238-1249,12.DOI:10.19756/j.issn.0253-3820.251026
金纳米颗粒负载多金属氧酸盐纳米带类过氧化物酶用于灭线磷的比色检测
Peroxidase-like Nanozyme Based on Gold Nanoparticle Supported Polyoxometalate Nanoribbons for Colorimetric Detection of Organophosphorus Pesticide Ethoprophos
摘要
Abstract
Organophosphorus pesticides(OPs)are widely used in global agriculture,and pose a serious threat to ecological environment and human health due to their high environmental persistence and biological toxicity.Colorimetric sensing strategies based on the inhibition of acetylcholinesterase(AChE)have become an important method for detecting OPs because of their simplicity and high specificity.However,the sensitivity is limited by the insufficient catalytic efficiency of traditional nanozymes.In this study,a one-step solvothermal method was used to synthesize polyoxometalate nanoribbons(POM)loaded with gold nanoparticles(Au NPs),named Au-POM.Experimental results showed that Au-POM could catalyze the decomposition of H2O2 in an acidic environment(pH 4.0),demonstrating typical peroxidase-like activity.Based on this,an AChE,choline oxidase(ChOx)and Au-POM nano enzyme cascade catalytic system was constructed.In this system,AChE specifically catalyzed the hydrolysis of acetylcholine(ACh)to choline,and then ChOx mediated the oxidation of choline to produce H2O2.During this process,Au-POM acted as a peroxidase-like enzyme to catalyze the decomposition of H2O2 to generate reactive oxygen species,triggering a specific oxidation reaction of the chromogenic substrate 3,3',5,5'-tetramethylbenzidine(TMB)into oxidized form.When the OP pesticide ethoprophos(EP)was present,it inhibited the activity of AChE and blocked the generation of ACh and H2O2,indirectly inhibiting the oxidation of TMB.The color and absorbance of the solution changed in a concentration-dependent manner.The detection limit of this method for EP was 1.05 μmol/L,and the linear response range was 20-180 μmol/L(R2=0.998).This method was applied to detection of environmental water samples and coriander samples with satisfactory results,providing a reliable technical platform for monitoring of OPs in environment and food.关键词
多金属氧酸盐/金纳米颗粒/纳米酶/有机磷/比色检测Key words
Polyoxometalate/Gold nanoparticles/Nanozyme/Organophosphate/Colorimetric detection引用本文复制引用
王琪,王依婷,臧浩,王强,甄淑君..金纳米颗粒负载多金属氧酸盐纳米带类过氧化物酶用于灭线磷的比色检测[J].分析化学,2025,53(8):1238-1249,12.基金项目
国家自然科学基金项目(No.22322409)资助. Supported by the National Natural Science Foundation of China(No.22322409). （No.22322409）
