分析化学2025，Vol.53Issue(8)：1300-1310,11.DOI:10.19756/j.issn.0253-3820.251142
高灵敏比率型表面增强拉曼基底用于快速分析血清中的6-硫鸟嘌呤
High-sensitivity Ratio-type Surface-enhanced Raman Substrate for Rapid Quantitative Determination of 6-Thioguanine in Serum
摘要
Abstract
6-Thioguanine(6-TG)is an antineoplastic agent used in treatment of acute leukemia.However,significant interindividual variability in dosing regimens and frequent clinical manifestations of hepatotoxicity and myelosuppression as adverse effects have affected its therapeutic efficacy.Consequently,the development of rapid analytical methods for 6-TG in clinical samples,enabling continuous therapeutic drug monitoring of plasma concentrations,holds substantial significance in optimizing dosage regimens,mitigating adverse reactions,and investigating drug metabolism mechanisms.In this study,multi-tipped gold nanostars(AuNSs)were prepared.With bis-(p-sulfonylphenyl)phenylphosphine molecule as the protecting agent and internal standard molecule,the AuNSs were assembled onto a highly sensitive surface-enhanced Raman(SERS)substrate for developing a ratio-based SERS quantitative analysis method for 6-TG in serum.The AuNSs containing multiple tips and gaps exhibited strong local surface plasmon resonance effect and SERS activity,ensuring the sensitivity of the analytical method.Furthermore,the introduction of internal standard molecules could improve the reproducibility,which guaranteed this method suitable for rapid analysis of drug molecules in complex samples.Quantitative analysis of 6-TG was achieved with linear detetion range of 1.0×10‒4-1.0 mmol/L.In the spiked recovery experiments of serum,the RSD was less than 5.32%,and the recoveries were 94%-104%,which proved that this method could be used for rapid quantitative determination of 6-TG in serum.This method provided a powerful tool for studying drug pharmacokinetics,which could promote the optimization of the usage methods of anti-cancer drugs,and it was expected to further enhance the clinical efficacy and safety of 6-TG,enabling it to achieve the best therapeutic effect.关键词
比率型表面增强拉曼基底/金纳米星/6-硫鸟嘌呤/抗癌药物Key words
Ratio-type surface-enhanced Raman substrate/Gold nanostars/6-Thioguanine/Anti-cancer drug引用本文复制引用
刘炎彬,韩艺超,王蓉,吴晓梅,王琴,姚媛媛,王悦靓,郭隆华..高灵敏比率型表面增强拉曼基底用于快速分析血清中的6-硫鸟嘌呤[J].分析化学,2025,53(8):1300-1310,11.基金项目
国家自然科学基金项目(Nos.22204062,22074054)、浙江省高层次人才特殊支持计划项目(No.2021R52044)、浙江省自然科学基金项目(Nos.LY24B050002,ZCLTGN24B0501,LQ23B050002)和浙江省教育厅一般研究项目(No.Y202352088)资助. Supported by the National Natural Science Foundation of China(Nos.22204062,22074054),the Zhejiang Provincial High-level Talent Special Support Plan(No.2021R52044),the Nature Sciences Funding of Zhejiang Province(Nos.LY24B050002,ZCLTGN24B0501,LQ23B050002)and the General Research Project of Education Department of Zhejiang Province(No.Y202352088). （Nos.22204062,22074054）
