分析化学2025，Vol.53Issue(8)：1320-1330,中插88-中插92,16.DOI:10.19756/j.issn.0253-3820.241446
特征离子导向策略结合高分辨质谱技术在保健品二氟拉松类成分测定及靶向筛查中的应用研究
Research on Targeted Screening of Diflorasone Components in Health Products Using Feature Ion Guided Strategy Combined with High-Resolution Mass Spectrometry
摘要
Abstract
A method for determination and targeted screening of diflorasone components in health products using ultra performance liquid chromatography-quadrupole time of flight mass spectrometry(UPLC-Q-TOF/MS)was established.Four representative diflorasone and esters(diflorasone,diflorasone diacetate,diflorasone-17-propionate,and diflorasone-21-propionate)were selected to optimize the pretreatment conditions,and 10 mL of extraction solvent dosage,15 min of extraction time and 5 g of salting-out agent as the optimal conditions were selected by response surface methodology.The results showed that the four analytes exhibited good linearity within the concentration range of 2.0‒100 μg/L with the chromatographic peak area,and the correlation coefficients(R2)were all greater than 0.9990,while the results of recovery and relative standard deviation could satisfy the requirements of determination.The common characteristic ions of diflorasone and esters werem/z121 andm/z335,and their specific structures were obtained by analyzing the cleavage pathway based on the optimized determination conditions.A targeted screening method for other esters of diflorasone based on characteristic ions guidance strategy was established.This method had many advantages such as high efficiency,high sensitivity and good reproducibility,and could be used for targeted screening of diflorasone and esters in health products.The developed characteristic ion guided strategy could be employed to construct mass spectral databases for various glucocorticoids,enabling comprehensive targeted screening across a broad range of compounds.关键词
特征离子导向策略/高分辨质谱/二氟拉松/响应曲面设计/保健品Key words
Feature ion guided strategy/High resolution mass spectrometry/Diflorasone/Response surface design/Health products引用本文复制引用
区硕俊,林茵茵,章海涛,岑建斌,王志元,郭新东,张嘉俊,梁志森,曾广丰..特征离子导向策略结合高分辨质谱技术在保健品二氟拉松类成分测定及靶向筛查中的应用研究[J].分析化学,2025,53(8):1320-1330,中插88-中插92,16.基金项目
海关总署科技计划项目(Nos.2019HK114,2024HK085)资助. Supported by the Science&Technology Planning of General Administration of Customs of China(Nos.2019HK114,2024HK085). （Nos.2019HK114,2024HK085）
