工业微生物2025，Vol.55Issue(4)：17-20,4.DOI:10.3969/j.issn.1001-6678.2025.04.006
基于GO/Fe3O4磁性纳米材料制备高效降解PE的交联酶制剂研究
Research on the Preparation of Cross-linked Enzyme Preparation for the Efficient Degradation of PE Based on GO/Fe3O4 Magnetic Nanocomposites
摘要
Abstract
In this paper,the GO/Fe3O4 magnetic nanocomposites was used as carriers to immobilize key enzymes for PE degradation to prepare a novel enzyme preparation.The optimal parameters for the preparation of magnetic carrier graphene oxide were as follows:reaction in an ice water bath for 2 hours,reaction in a 40℃water bath for 2 hours,reaction in a 98℃water bath for 5 minutes,and freeze-drying treatment.When the carrier was covalently crosslinked with PE degradation complex enzyme at a mass ratio of 99∶1,the enzyme immobilization rate reached 100%.After 5 days of degradation by cross-linking enzyme preparation,the PE degradation rate reached 4.5%.关键词
交联酶制剂/PE/磁性纳米载体/降解率Key words
cross-linked enzyme preparation/PE/magnetic nanocarrier/degradation rate引用本文复制引用
王欢莉,丁嘉骏,杨昆妍,赵雅婷,苏凯越,王加连..基于GO/Fe3O4磁性纳米材料制备高效降解PE的交联酶制剂研究[J].工业微生物,2025,55(4):17-20,4.基金项目
江苏省大学生创新创业计划项目"高效降解聚乙烯(PE)的生物酶制剂制备及其应用效果研究"(编号:202210324215H). （PE）
