工业微生物2025，Vol.55Issue(4)：33-37,51,6.DOI:10.3969/j.issn.1001-6678.2025.04.010
酶结构中通道分析与改造的研究进展
Advances in the Analysis and Modification of Channels in Enzyme Structures
摘要
Abstract
The rational design of enzymes has mainly focused on the active center and its surrounding area.However,in recent years,the research has gradually extended to other peripheral residues and even distal residues.The channels in enzymes,which connect the external solvent to the enzyme's active site,significantly influence catalytic activity,substrate specificity,and stereoselectivity of enzymes.Consequently,the peripheral amino residues constituting these channels have become a focus in rational design.The paper introduces methods for identifying and analyzing the channels in enzyme structures,reviews representative cases of enzyme channel modification,and explores the structure-function relationship between channel architecture and catalytic functionality in enzymes.关键词
酶通道分析/酶改造/理性设计/分子动力学模拟Key words
channel architecture analysis/enzyme modification/rational design/molecular dynamic simulation引用本文复制引用
刘璐,杨志钧,邵雷,吴锴..酶结构中通道分析与改造的研究进展[J].工业微生物,2025,55(4):33-37,51,6.基金项目
上海市科学技术委员会"科技创新行动计划"扬帆项目(20YF1417900). （20YF1417900）
