河南农业科学2025，Vol.54Issue(9)：53-60,8.DOI:10.15933/j.cnki.1004-3268.2025.09.006
烟草NtWRKY65基因在响应低氮胁迫中的功能分析
Functional Analysis of NtWRKY65 Gene in Tobacco under Low Nitrogen Stress
摘要
Abstract
In order to investigate the function of tobacco NtWRKY65 gene in response to low nitrogen stress,wild-type K326(WT)and four transgenic lines with overexpressing NtWRKY65(OE)were used as materials,and the differences in phenotype,root traits,nitrogen metabolism related enzyme activity,nitrogen metabolism and aging related gene expression levels of different materials under low nitrogen stress and normal nitrogen supply conditions were studied by using hydroponic methods.The results showed that compared with WT plants,the main root length and lateral root number of OE plants significantly increased,and the dry matter mass and total nitrogen content significantly increased by 21.83%—45.12%and 94.65%—271.57%,respectively under low nitrogen stress.The growth potential of OE plants was significantly enhanced.Under low nitrogen stress,the activities of key enzymes for nitrogen absorption and assimilation(nitrate reductase,glutamate dehydrogenase),as well as the key enzyme for nitrogen redistribution(glutamine synthetase),and the expression levels of related genes(NtNR,NtGS1-3,NtGS2,NtGDH1)in OE plants were significantly increased compared to WT plants.The expression levels of mature aging related genes NtMC,NtCP1,NtCP23,and NtCCD in the leaves of OE plants were significantly reduced compared to WT plants under low nitrogen stress.These results preliminarily confirm that overexpression of NtWRKY65 could promote root development in tobacco plants,enhance their ability to absorb,transport,and assimilate nitrogen under low nitrogen stress,inhibit leaf senescence,and positively regulate tobacco tolerance to low nitrogen stress.NtWRKY65 could be used as a candidate gene resource for genetic improvement of tobacco nitrogen use efficiency.关键词
烟草/NtWRKY65/过表达/低氮胁迫/功能验证Key words
Tobacco/NtWRKY65/Overexpression/Low nitrogen stress/Functional validation分类
农业科技引用本文复制引用
李朝阳,王瑞刚,贾振宇,张玉宁,李洪臣,薛松格,陈海洋,李丽华,张小全..烟草NtWRKY65基因在响应低氮胁迫中的功能分析[J].河南农业科学,2025,54(9):53-60,8.基金项目
河南省科技攻关项目(242102110288) （242102110288）
河南省烟草公司洛阳市公司科技项目(2024410300270126) （2024410300270126）
河南省烟草公司三门峡市公司科技项目(2024411200200016X) （2024411200200016X）
