郑州大学学报（医学版）2025，Vol.60Issue(5)：593-597,5.DOI:10.13705/j.issn.1671-6825.2024.11.066
肝细胞癌基因诊断标志物和免疫浸润标志物的分析和识别
Analysis and identification of biomarkers for diagnosis and immune infil-tration of hepatocellular carcinoma
摘要
Abstract
Aim:To screen the potential biomarkers for gene diagnosis and immune infiltration of hepatocellular carci-noma(HCC).Methods:Gene expression profile microarray data of both normal adjacent(NAT)and HCC tissues from GEO database were used to screen the highly differentially expressed gene(HDEG)of HCC through LASSO regression and ran-dom forest model.Data from TCGA database were used to draw the ROC curve of gene diagnosis of HCC using HDEG,and lower limit of AUC 95％CI＞0.8 was taken as the standard of identification of the potential biomarkers for gene diagnosis.The expression levels of target genes in 16 cases of HCC and paired NAT tissues from the Second Affiliated Hospital of Zhengzhou University were detected by qRT-PCR.The association between the target genes and 22 kinds of immune cells in-filtration characteristics were analyzed using single-sample gene set enrichment analysis algorithm based on GEO data.Re-sults:Apolipoprotein F(APOF),hepcidin antimicrobial peptide(HAMP),complement 9(C9)and metallothionein 1E(MT1E)were screened as potential gene diagnostic biomarkers for HCC,and the AUC(95％CI)were 0.953(0.933-0.973),0.946(0.922-0.970),0.926(0.901-0.951),0.925(0.899-0.950),respectively,and their expressions were all downregulated in HCC tissues compared with NAT(P＜0.05).The infiltration levels of γδ T cells,monocytes,M2 macrophages,activated mast cells and neutrophils decreased,and those of activated memory CD4 T cells,regulatory T cells and MO macrophages increased in HCC tissues(P＜0.05).APOF,HAMP,C9,and MT1E were correlated with the immune cells and function(P＜0.05).Conclusion:APOF,HAMP,C9 and MT1E may serve as potential gene diagnostic biomarkers and therapeutic targets for HCC.关键词
肝细胞癌/机器学习/生物标志物/免疫浸润Key words
hepatocellular carcinoma/machine learning/biomarker/immune infiltration分类
医药卫生引用本文复制引用
王子康,王龙飞,王克雷,方艳,刘超..肝细胞癌基因诊断标志物和免疫浸润标志物的分析和识别[J].郑州大学学报（医学版）,2025,60(5):593-597,5.基金项目
国家自然科学基金项目(82304615) （82304615）
河南省重点科技攻关项目(242102310239) （242102310239）
河南省医学科技攻关(联合共建)项目(LHGJ20230344) （联合共建）
河南省医学科技攻关计划省部共建重点项目(SBGJ202402071) （SBGJ202402071）
