郑州大学学报（医学版）2025，Vol.60Issue(5)：609-612,4.DOI:10.13705/j.issn.1671-6825.2024.06.081
帕博西尼对宫颈癌Hela细胞代谢组学的影响
Effect of palbociclib on metabolomics of cervical cancer Hela cells
摘要
Abstract
Aim:To investigate the effect of palbociclib on the metabolomics of cervical cancer Hela cells.Methods:Hela cells were treated with palbociclib at concentrations of 0.00,0.25,0.50,and 1.00 μmol/L for 24,48,and 72 hours,and cell proliferation was assessed using MTT assay.Cells treated with 0.00 and 0.50 μmol/L palbociclib for 72 hours were collected for metabolomic analysis via high-resolution liquid chromatography-mass spectrometry(HR-LCMS).The differenti-al metabolites between the 2 groups were compared and screened,and functional enrichment analysis of metabolic pathways was performed using the KEGG analysis.Results:Palbociclib significantly inhibited the proliferation of Hela cells,with the inhibitory effect enhancing in a dose-dependent manner(P＜0.05).HR-LCMS identified 39 cationic differential metabolites and 24 anionic differential metabolites.Enrichment analysis indicated that the primary cationic metabolic pathway was the dopaminergic synapse pathway,while the primary anionic metabolic pathway was fatty acid biosynthesis.Conclusion:Palbo-ciclib could inhibit the proliferation of Hela cells,and the mechanism may involve the inhibition of fatty acid biosynthesis and disruption of the dopaminergic synapse pathway.关键词
高分辨液相色谱质谱法/代谢组学/帕博西尼/Hela细胞Key words
high-resolution liquid chromatography-mass spectrometry/metabolomics/palbociclib/Hela cell分类
医药卫生引用本文复制引用
位冒冒,张成杰,信凯月,闫春蕊,韩卓航,徐耀瑜..帕博西尼对宫颈癌Hela细胞代谢组学的影响[J].郑州大学学报（医学版）,2025,60(5):609-612,4.基金项目
河南省含氟药物精准合成工程技术研究中心经费支持项目(192109521014) （192109521014）
安阳市科技攻关基金项目(2022C01SF089) （2022C01SF089）
安阳市科技特派员项目(202233) （202233）
