郑州大学学报（医学版）2025，Vol.60Issue(5)：632-636,5.DOI:10.13705/j.issn.1671-6825.2025.02.083
紧密连接蛋白12对胶质母细胞瘤细胞增殖和上皮间充质转化的影响
Effects of Claudin-12 on proliferation and epithelial-mesenchymal transi-tion of glioblastoma cells
摘要
Abstract
Aim:To investigate the effects of Claudin-12(CLDN12)on the proliferation and epithelial-mesenchymal transition(EMT)of glioblastoma(GBM)cells.Methods:Lentivirus infection was employed to construct CLDN12 low-ex-pression human GBM U87 and U251 cells.Cell viability was assessed by MTT assay.The expression of CLDN12 and cell proliferation markers(P1 6,P21,PCNA)at the mRNA and protein levels was detected by qRT-PCR and Western blot.The fluorescence intensity of EdU and E-cadherin was evaluated by immunofluorescence.EMT and PI3K-AKT signaling path-way-related proteins were detected by Western blot.Results:Compared with the negative control group,the viability of shCLDN12 group was significantly reduced at 72 and 96 hour-culture;the expression levels of P16 and P21 mRNA and pro-tein were significantly upregulated;PCNA mRNA and protein expression levels were downregulated;the protein expression levels of ZEB1,N-cadherin,and Vimentin were decreased;KRT 19 protein expression level was increased;the expressions of p-PI3K and p-AKT proteins were significantly inhibited(P＜0.05).Compared with the negative control group,the percent-age of EdU-positive cells in shCLDN12 group was significantly reduced,and the fluorescence intensity of E-cadherin was significantly increased(P＜0.05).Conclusion:CLDN12 may promote GBM cell proliferation and EMT by activating the PI3K-AKT signaling pathway.关键词
胶质母细胞瘤/紧密连接蛋白12/细胞增殖/上皮间充质转化/PI3K-AKTKey words
glioblastoma/Claudin-12/cell proliferation/epithelial-mesenchymal transition/PI3K-AKT分类
医药卫生引用本文复制引用
姚要兵,姚明辰,王志清,李丹阳,苑家畅,贾延劼..紧密连接蛋白12对胶质母细胞瘤细胞增殖和上皮间充质转化的影响[J].郑州大学学报（医学版）,2025,60(5):632-636,5.基金项目
河南省自然科学基金项目(232300420037) （232300420037）
