福建中医药2025，Vol.56Issue(6)：25-31,7.DOI:10.13260/j.cnki.jfjtcm.2025.06006
基于网络药理学和动物实验探讨血府逐瘀汤治疗外周动脉疾病肌肉损伤的作用机制
Mechanism of Xuefu Zhuyu Decoction in Treating Muscle Injury Induced by Peripheral Arterial Disease Based on Network Pharmacology and Animal Experiments
摘要
Abstract
Objective:To investigate the mechanism of Xuefu Zhuyu Decoction(XFZYD)in the treatment of muscle injury in-duced by peripheral arterial disease(PAD)using network pharmacology,molecular docking,and animal experiments.Methods:The TCMSP,HERB,and PubChem databases were searched along with literature mining to collect active ingredients and potential targets of XFZYD.PAD-related gene targets were obtained from the GEO datasets.The intersection of the two sets of targets was taken to identify potential therapeutic targets of XFZYD for PAD.Cytoscape 3.10.2 was used to construct a drug component-target-disease in-teraction network and identify key active ingredients.The STRING platform was employed to analyze potential target interactions and identify core targets.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed on the core targets.Molecular docking was used to analyze the binding affinity between core targets and key drug compo-nents.Twenty-four rats were randomly divided into four groups using a random number table:blank control group,model group,XFZYD conventional dose group,and XFZYD high dose group,with six rats in each group.After 7 days of prophylactic administra-tion,a rat model of lower limb ischemic muscle injury was established by femoral artery embolization with Bletilla striata micro-spheres.Tissue samples were collected 5 days after modeling.Hematoxylin-eosin(HE)staining was used to observe the morphologi-cal structure of the gastrocnemius muscle in all four groups.Serum levels of lactate dehydrogenase(LDH)and nitric oxide(NO)were measured.Immunohistochemistry was used to detect CYP1B1 expression in the gastrocnemius muscle.Results:Network phar-macology analysis predicted 198 active ingredients in XFZYD.Ferulic acid and amygdalin were identified as potential core active components for the treatment of PAD,and cytochrome P450 family member CYP1B1 was predicted to be a key drug target.Animal experiments showed that compared with the blank control group,the model group exhibited inflammatory cell infiltration and in-creased intermuscular space in gastrocnemius muscle tissue,increased serum LDH levels(P<0.05),decreased serum NO levels(P<0.05),and reduced CYP1B1 protein expression in ischemic gastrocnemius tissue(P<0.05).Compared with the model group,both the XFZYD conventional dose group and the XFZYD high dose group showed improved gastrocnemius muscle morphology,reduced in-termuscular space,decreased serum LDH levels(P<0.05),increased serum NO levels(P<0.05),and increased CYP1B1 protein ex-pression in ischemic gastrocnemius tissue(P<0.05).Conclusion:XFZYD can promote the recovery of the gastrocnemius muscle in rats with lower limb ischemia,and its mechanism may be associated with CYP1B1.关键词
外周动脉疾病/肌肉损伤/血府逐瘀汤/网络药理学/分子对接/CYP1B1Key words
peripheral arterial disease/muscle injury/Xuefu Zhuyu Decoction/network pharmacology/molecular docking/CYP1B1引用本文复制引用
平春佳,俞嘉明,程烨,张嘉愉,符娟丽,左春兰,林凡..基于网络药理学和动物实验探讨血府逐瘀汤治疗外周动脉疾病肌肉损伤的作用机制[J].福建中医药,2025,56(6):25-31,7.基金项目
国家自然科学基金项目(82074191,82204969) （82074191,82204969）
福建省自然科学基金项目(2024J01748) （2024J01748）
福建中医药大学校管科研课题(X2023009) （X2023009）
