昆明医科大学学报2025，Vol.46Issue(8)：10-19,10.DOI:10.12259/j.issn.2095-610X.S20250802
虫生真菌Samsoniella hepiali CDB9-31化学表观遗传修饰研究
Studies on the Chemical Epigenetic Modification of Fungus Samsoniella Hepiali CDB9-31
摘要
Abstract
Objective To analyze the effects of the histone deacetylase inhibitor suberoylanilide hydroxamic acid(SAHA)on the secondary metabolites of the entomopathogenic fungus Samsoniella hepiali CDB9-31 using thin-layer chromatography(TLC)and high-performance liquid chromatography(HPLC).Methods The fermentation products of Samsoniella hepiali CDB9-31 treated with epigenetic modifiers were separated and purified using methods such as silica gel column chromatography,Sephadex column chromatography and reversed-phase column chromatography.The structures of the compounds were elucidated using modern spectroscopic analysis methods.The antimicrobial activity of the obtained monomeric compounds was determined using the filter paper disc diffusion method.Results The TLC and HPLC analyses of its fermentation extracts revealed that SAHA could induce the strain to produce more diverse array of secondary metabolites,and 11 monomeric compounds were isolated and identified as follows:N'-phenyloctanediamide(1),5-Phenylcarbamoyl-pentanoic(2),ergosterol(3),5,8-Epidioxy-5α,8α-ergosta-6,22E-diene-3β-ol(4),1-monolinolein(5),(4E,8E)-2-N-(2-Hydro-xypalmitoyl)-1-O-(β-D-glucopyranosyl)-9-methyl-4,8-sphingadienine(6),Ergosterol peroxide 3-O-β-D-glucopyranoside(7),(22E,24R)-7,22-diene-3β,5α,6β-ergostatriol(8),(2S,2'R,3R,4E,8E)-N-2'-Hydroxyhexadecanoyl-2-amion-9-methyl-4,8-octadecadiene-1,3-diol(9),Adenosine(10),D-Glulopyranose(11).Compounds 1 and 2 were derivatives of SAHA,and it was speculated that the special metabolic environment of CDB9-31 caused the biotransformation of SAHA.Except for compound 3,all other compounds were isolated from this genus for the first time.The antibacterial activity results showed that six of these compounds exhibited varying degrees of inhibitory effects against at least one pathogenic bacterium.Conclusion This study has enriched the chemical diversity of secondary metabolites from the entomopathogenic fungus Samsoniella hepiali.关键词
表观遗传修饰/虫生真菌/Samsoniella hepiali/次生代谢产物/抗菌活性Key words
Epigenetic modification/Entomopathogenic fungi/Samsoniella hepiali/Secondary metabolites/Antimicrobial activity分类
医药卫生引用本文复制引用
赵金丽,阳红燕,杨佳倩,陈馨忆,赵庆,张晓梅..虫生真菌Samsoniella hepiali CDB9-31化学表观遗传修饰研究[J].昆明医科大学学报,2025,46(8):10-19,10.基金项目
国家自然科学基金(82360697) （82360697）
云南省科技计划项目(202101AZ070001-204) （202101AZ070001-204）
