临床误诊误治2025，Vol.38Issue(17)：70-74,5.DOI:10.3969/j.issn.1002-3429.2025.17.015
LASP1调节甲状旁腺激素相关蛋白对未分化甲状腺癌细胞增殖及凋亡的影响
Effect of LASP1 on proliferation and apoptosis of undifferentiated thyroid cancer cells by regulating parathyroid hormone-related proteins
摘要
Abstract
Objective To investigate the effect of LIM and SH3 protein 1(LASP1)on the proliferation and apoptosis of undifferentiated thyroid cancer cells by regulating parathyroid hormone-associated protein(PTHRP).Methods Immunohistochemical staining and Western blotting were used to analyze the expression level of LASP1 in undifferentiated thyroid cancer tissues and adjacent tissues.The undifferentiated thyroid carcinoma Hth74 cells were divided into the si NC group,the si LASP1 group and the si PTHRP group.The proliferation activity of Hth74 cells was analyzed by CCK-8 assay and cell colony formation assay,and the apoptosis rate of Hth74 cells was analyzed by in situ end-labeling method.The expression levels of LASP1 and PTHRP in Hth74 cells were detected by Western blotting.Results The expression level of LASP1 in cancer tissues was higher than that in adjacent tissues(P<0.01).Compared with the si NC group,the proliferation ability of Hth74 cells in the si LASP1 group and the si PTHRP group was decreased(P<0.05).Compared with the si NC group,the apoptosis rate of Hth74 cells in the si LASP1 group and the si PTHRP group was increased(P<0.05).Compared with the si NC group,the expressions of LASP1 and PTHRP in the si LASP1 group were both decreased,and the expression of PTHRP in the si PTHRP group was decreased(P<0.05).Conclusion In undifferentiated thyroid cancer tissues,the expression of LASP1 is increased,and the expression of PTHRP is decreased after inhibiting LASP1.Reducing the expression of LASP1 or PTHRP can inhibit the proliferation of undifferentiated thyroid cancer cells and promote its apoptosis.关键词
未分化甲状腺癌/LIM和SH3蛋白1/甲状旁腺激素相关蛋白/细胞凋亡/Hth74细胞/细胞增殖Key words
undifferentiated thyroid carcinoma/LIM and SH3 proteins 1/parathyroid hormone-related protein/apoptosis/Hth74 cells/cell proliferation引用本文复制引用
白图布心,丁晓丽,李青平,兰丽丽,沈广泰,何和平,张志鹏,张玉海,田瑞博,于晓强,孙天扬,侯天..LASP1调节甲状旁腺激素相关蛋白对未分化甲状腺癌细胞增殖及凋亡的影响[J].临床误诊误治,2025,38(17):70-74,5.基金项目
内蒙古自治区科技计划项目(2022YFSH0106) （2022YFSH0106）
