现代畜牧兽医Issue(8)：55-59,5.DOI:10.20154/j.cnki.issn1672-9692.2025.08.010
陆基圆池循环水条件下投饲率对大口黑鲈肝脏组织结构、免疫功能的影响
Effects of feeding rate on liver histology and immune function of Micropterus salmoides under land-based circular-pond recirculating aquaculture systems
摘要
Abstract
This study aimed to investigate the effects of different feeding rates on serum lipid levels,liver antioxidant capacity,immune function,and histology of Micropterus salmoides in a land-based circular-pond recirculating aquaculture systems,in order to determine the optimal feeding strategy.The experiment selected Micropterus salmoides with a body weight of(23.31±1.25)g as the research subjects and randomly divided them into four groups(1.5%group,2.5%group,3.5%group,and 4.5%group)according to the feeding rate.Two replicates were set in each group,and each replicate was raised in one experimental pool,with 3 660 bass(73 bass/m2)released in each pool.After 7 days of temporary rearing,30 bass were randomly selected from each pool to measure their body weight,and the average daily feeding amount for each group was calculated.The test period is 60 days.The results showed that compared with the 1.5%and 2.5%groups,a feeding rate of 4.5%could significantly increase the contents of serum triglycerides(TG)and total cholesterol(TC)in Micropterus salmoides(P<0.05).Compared with the 1.5%and the 4.5%groups,feeding rates of 2.5%and 3.5%could significantly increase the content of reduced glutathione(GSH)in the liver of Micropterus salmoides,and feeding rate of 2.5%could significantly increase the activity of catalase(CAT)in the liver of Micropterus salmoides(P<0.05).Compared with the 4.5%group,a feeding rate of 2.5%could significantly increase the lysozyme(LZM)activity in the liver of Micropterus salmoides(P<0.05).Feeding rates of 1.5%and 2.5%could significantly increase the activity of superoxide dismutase(SOD)in the liver of Micropterus salmoides(P<0.05),and feeding rates of 1.5%,2.5%,and 3.5%could significantly reduce the content of malondialdehyde(MDA)in the liver of Micropterus salmoides(P<0.05).Compared with the 1.5%and 2.5%groups,the feeding rates of 3.5%and 4.5%could significantly increase the long diameter,short diameter,nucleus diameter and vacuolar area ratio of hepatocytes of Micropterus salmoides,and significantly reduce the nucleus area ratio(P<0.05).Compared with the 1.5%group,feeding rates of 3.5%and 4.5%could significantly reduce the number of nuclei in hepatocytes of Micropterus salmoides(P<0.05).Liver tissue sections showed that as the feeding rate increased,the fat accumulation in the liver of Micropterus salmoides gradually increased.When the feeding rate reached 4.5%,liver cell swelling and fat vacuolation were more severe.Studies have shown that a feeding rate of 2.5%to 3.5%helps enhance the antioxidant capacity and immune function of Micropterus salmoides,as well as maintain the integrity of liver tissue structure.关键词
陆基圆池/大口黑鲈/投饲率/抗氧化功能/免疫功能/肝脏组织结构Key words
Land-based circular-pond/Micropterus salmoides/Feeding rate/Antioxidant function/Immune function/Liver tissue structure分类
农业科技引用本文复制引用
韦云勇,王梦然,刘宏祥,沈佳佳,高风,程光平,麻艳群..陆基圆池循环水条件下投饲率对大口黑鲈肝脏组织结构、免疫功能的影响[J].现代畜牧兽医,2025,(8):55-59,5.基金项目
广西重点研发计划项目(桂科AB21220019) （桂科AB21220019）
