现代畜牧兽医Issue(8)：60-64,5.DOI:10.20154/j.cnki.issn1672-9692.2025.08.011
羊驼源弗格森埃希菌的分离鉴定和药敏试验
Isolation,identification,and antimicrobial susceptibility test of Escherichia fergusonii from alpacas
摘要
Abstract
A case of abortions,weak births,and neonatal mortality in alpacas occurred on a farm in Shanxi Province.This study aimed to identify the causative pathogen and provide diagnostic and disease control strategies for the farm.Heart,liver,spleen,and kidney samples from deceased alpacas were aseptically collected and inoculated onto multiple culture media,for bacterial isolation.Colony growth was observed,and single colonies were subjected to Gram staining for morphological analysis.The isolates were further identified by PCR and antimicrobial susceptibility testing.Results showed bacterial growth on all media.Colonies appeared pink on MacConkey and SS agar,while small colonies with metallic sheen were observed on eosin methylene blue(EMB)agar.Microscopic examination revealed abundant uniform Gram-negative bacilli.These morphological characteristics aligned with Escherichia fergusonii.PCR amplification,sequencing,and BLAST analysis confirmed the isolates as Escherichia fergusonii.Antimicrobial susceptibility testing indicated that the isolates were susceptible to fluoroquinolones,aminoglycosides,cephalosporins,azithromycin,and florfenicol.The study identified Escherichia fergusonii as the causative pathogen responsible for the alpaca infections on this farm,demonstrating susceptibility to fluoroquinolones,aminoglycosides,cephalosporins,azithromycin,and florfenicol.关键词
羊驼/弗格森埃希菌/流产/腹泻/PCR鉴定Key words
Alpaca/Escherichia fergusonii/Abortion/Diarrhea/PCR identification分类
农业科技引用本文复制引用
刘华栋,路自涵,王瑞,韩欣润,李俊,李婷婷,王国艳,唐娟..羊驼源弗格森埃希菌的分离鉴定和药敏试验[J].现代畜牧兽医,2025,(8):60-64,5.基金项目
山西七峰山农牧科技有限公司合作项目(202273) （202273）
