器官移植2025，Vol.16Issue(5)：747-755,9.DOI:10.12464/j.issn.1674-7445.2025150
基因修饰猪-猴异种心脏移植模型免疫反应动态特征分析
Dynamic analysis of immune responses in heterotopic heart transplantation model of genetically modified pig-to-macaque
摘要
Abstract
Objective To evaluate the efficacy of a combined immunosuppression regimen in modulating rejection in genetically modified pig-to-macaque xenogeneic heart transplantation.Methods Two xenogeneic heart transplantation models were constructed using genetically modified pigs and macaques.Dynamic monitoring of recipient peripheral blood immune parameters and observation of graft pathological changes were performed.Results Regimen 1,featuring B-cell depletion,T-cell inhibition,and C3 complement suppression,reduced lymphocyte levels but failed to control acute humoral rejection and macrophage infiltration.Regimen 2,adding C5 complement inhibition and interleukin-6 inhibition to Regimen 1,more effectively lowered lymphocyte levels,inhibited acute humoral rejection and complement activation,and decreased antibody deposition.However,a late-phase cytokine storm and residual T cells emerged.Conclusions Regimen 2 reduces the hyperacute and acute rejection risks through multi-target intervention.Yet,it requires balancing medication complexity and safety.This indicates the need to optimize cellular immune regulation and adjust the plan through dynamic multidimensional monitoring.关键词
基因修饰猪/恒河猴/异种心脏移植/免疫抑制药/补体/细胞因子/免疫细胞/共刺激通路Key words
Genetically modified pig/Macaque/Xenogeneic heart transplantation/Immunosuppressant/Complement/Cytokine/Immune cell/Co-stimulatory pathway分类
医药卫生引用本文复制引用
白乐,戴自强,任志鹏,赖成虹,李先华,谢晓阳,潘登科,龙恩武,李巅远..基因修饰猪-猴异种心脏移植模型免疫反应动态特征分析[J].器官移植,2025,16(5):747-755,9.基金项目
国家重点研发计划(2024YFC3406804) （2024YFC3406804）
苏州市姑苏卫生人才计划(GSW2022065) （GSW2022065）
苏州市立医院"科教兴卫"专项基金(Szslyyzx2024001) （Szslyyzx2024001）
