中国森林病虫2025，Vol.44Issue(5)：1-10,10.DOI:10.19688/j.cnki.issn1671-0886.20250025
马尾松毛虫两个OBPs的表达分析及与其寄主挥发物的分子对接
Expression analysis of two odorant binding proteins from Dendrolimus punctatus and molecular docking with the host volatiles
摘要
Abstract
To elucidate the role of odorant binding proteins(OBPs)of Dendrolimus punctatus in host volatiles recognition,cDNA of two OBPs genes,DpunOBP6 and DpunOBP27,which were highly expressed in D.punctatus,were cloned using PCR,and the expression characteristics were determined by RT-qPCR;the evolutionary relationship and sequence structural characteristics were determined by using phylogenetic and sequence analyses,respectively;binding effects of the two proteins to the host volatiles were predicted by protein modeling and molecular docking simulations.The results demonstrated that relative expression levels of both DpunOBP6 and DpunOBP27 were low in eggs and pupae of D.punctatus,but reaching the highest level in antennae of adults.The three-dimensional structural model of DpunOBPs was obtained through protein modeling,and DpunOBP6 and DpunOBP27 had good binding effects with main volatile compounds of Pinus massoniana by molecular docking technology.Among 27 tested volatile compounds from P.massoniana,DpunOBP6 exhibited the lowest binding energy with four compounds:(+)-sativene,β-caryophyllene,ylangene and trans-caryophyllene.DpunOBP27 showed the lowest binding energy with β-farnesene.Molecular interaction analysis revealed that both DpunOBP6 and DpunOBP27 protein formed hydrogen bonding interactions with α-terpineol,and DpunOBP27 specifically formed hydrogen bonds with isopino-camphone.These findings provided a foundation for elucidating functions of gene DpunOBP6 and DpunOBP27,and for screening behavior-regulating agents of D.punctatus based on reverse chemical ecology.关键词
马尾松毛虫/气味结合蛋白/表达分析/蛋白质建模/分子对接Key words
Dendrolimus punctatus/odorant binding protein/expression analysis/protein modeling/molecular docking分类
农业科技引用本文复制引用
谢明慧,陈静,王正,李承锦,武承旭,张苏芳..马尾松毛虫两个OBPs的表达分析及与其寄主挥发物的分子对接[J].中国森林病虫,2025,44(5):1-10,10.基金项目
国家自然科学基金面上项目"马尾松毛虫适应寄主萜烯类防御物质的分子机制"(32371895) （32371895）
中国林业科学研究院基本科研业务费专项资金"重要林业害虫RNA干扰效率限速机制研究"(CAFYBB2020QC001) （CAFYBB2020QC001）
