现代检验医学杂志2025，Vol.40Issue(5)：35-39,5.DOI:10.3969/j.issn.1671-7414.2025.05.007
胆管癌患者血清circRNA MBOAT2,circRNA ACTN4水平表达与临床病理特征及预后的关系研究
Study on the Relationship between the Expression of Serum circRNA MBOAT2 and circRNA ACTN4 Levels and Clinicopathological Features and Prognosis in Patients with Cholangiocarcinoma
摘要
Abstract
Objective To investigate the expression changes of serum circRNA membrane bound O-acyltransferase 2(circRNA MBOAT2)and circRNA recombinant actinin 4(circRNA ACTN4)in patients with cholangiocarcinoma and their relationship with prognosis.Methods 96 patients with cholangiocarcinoma treated at the First Hospital of Handan City from January 2020 to January 2022 in Department of Hepatobiliary Surgery were regarded as as the cholangiocarcinoma group.Additionally,85 patients with intraductal stones and 90 healthy volunteers were collected as the stone group and control group,respectively.Quantitative real-time reverse transcription PCR(qRT-PCR)was applied to detect the expression levels of circRNA MBOAT2 and circRNA ACTN4.χ2 test was applied to analyze the relationship between circRNA MBOAT2 and circRNA ACTN4 and clinical pathological features.Pearson was applied to analyze the correlation between circRNA MBOAT2 and circRNA ACTN4 in patients with cholangiocarcinoma.ROC was applied to analyze the diagnostic value of circRNA MBOAT2 and circRNA ACTN4 in the occurrence of cholangiocarcinoma.COX was applied to analyze the influencing factors of poor prognosis in patients with cholangiocarcinoma.Kaplan-Meier method was applied for survival analysis.Results circRNA MBOAT2(1.24±0.38)and circRNA ACTN4(1.27±0.42)in cholangiocarcinoma group were higher than those in stone group(1.02±0.31,1.05±0.34)and control group(0.83±0.24,0.78±0.21),and the stone group was higher than that in control group,with statistically significance(t=5.016～14.025,all P<0.05).There was a positive correlation between circRNA MBOAT2 and circRNA ACTN4 in patients with cholangiocarcinoma group(r=0.428,P<0.05).Combined diagnosis of MBOAT2 and ACTN4 was better than single diagnosis of cholangiocarcinoma(Z=4.063,4.004,all P<0.05);circRNA MBOAT2 and circRNA ACTN4 were correlated with clinical staging and lymph node status(t=5.091～5.984,all P<0.05).Positive lymph node status and elevated levels of circRNA MBOAT2 and circRNA ACTN4 were independent risk factors for mortality(HR=1.527,1.582,1.727,all P<0.05).The cumulative survival rate of patients with high expression of circRNA MBOAT2(23.40%vs 46.94%)and circRNA ACTN4(24.00%vs 47.83%)was lower than that of patients with low expression(χ2=5.809,5.946,all P<0.05).Conclusion The serum levels of circRNA MBOAT2 and circRNA ACTN4 increase with the progression of the disease,and the combination of the two has certain value in diagnosing the occurrence of cholangiocarcinoma.关键词
胆管癌/环状RNA膜结合O-酰基转移酶2/环状RNA辅肌动蛋白α4/诊断价值/病理特征Key words
cholangiocarcinoma/circular RNA membrane-bound O-acyltransferases 2/circRNA actinin 4/diagnostic value/pathological features分类
医药卫生引用本文复制引用
李红磊,谢亚玎,伍杰,谭诚,张会娟..胆管癌患者血清circRNA MBOAT2,circRNA ACTN4水平表达与临床病理特征及预后的关系研究[J].现代检验医学杂志,2025,40(5):35-39,5.基金项目
2022年度河北省医学科学研究课题计划(编号:20220483). （编号:20220483）
