现代检验医学杂志2025，Vol.40Issue(5)：46-51,6.DOI:10.3969/j.issn.1671-7414.2025.05.009
2型糖尿病肾病患者外周血差异表达circRNA筛选及circAKT3水平表达与肾纤维化的相关性研究
Study of circRNA Screening for Differential Expression in Peripheral Blood and Correlation between CircAKT3 Level Expression and Renal Fibrosis in Patients with Type 2 Diabetic Nephropathy
摘要
Abstract
Objective To screen the differentially expressed circular RNA(circRNA)in peripheral blood of patients with type 2 diabetic nephropathy(T2DN)and study the correlation between the expression of circAKT3 level and renal fibrosis.Methods A retrospectively study was conducted on 176 T2DM patients admitted to Mianyang 404 Hospital from December 2022 to December 2023 based on whether the patients developed DN,they were divided into a simple T2DM group(n=60)and DN group(n=116).At the same time,30 healthy subjects who underwent physical examination in Mianyang 404 Hospital during the same period were selected as the control group.Clinical data of all subjects were collected.DN patients were divided into microalbuminuria group(M-DN group,n=66)and macroalbuminuria group(MS-DN group,n=50)according to Mogensen classification criteria.Twenty samples were randomly selected from the control group,M-DN group,T2DM group and MS-DN group,and 2 ml peripheral venous blood was extracted for RNA extraction and circRNA microarray scanning analysis to screen the differentially expressed circRNA.The correlation between the most significant differential circRNA expression level and renal fibrosis markers and renal function indicators was statistically analyzed.Receiver operator characteristic(ROC)curve was used to analyze the diagnostic efficacy of circAKT3 for DN.Results Compared with the control group,the level of circAKT3 in MS-DN patients was down-regulated by 9.72 times,which was the most significantly down-regulated circRNA.qRT-PCR showed that compared with the control group,the relative expression of circAKT3 in the T2DM group,M-DN group and MS-DN group was 0.85±0.23,0.54±0.13 and 0.12±0.09,respectively,with the progression of DN,the relative expression level of circAKT3 in peripheral blood of patients gradually decreased,and the differences were statistically significant(t=5.100,8.568,10.960,all P＜0.001).Spearman correlation test showed that the level of circAKT3 in peripheral blood of DN patients was correlated with the duration of diabetes,serum Creatinine,BUN,cystatin C,TGF-β,CIV,PCIII and Alb were negatively,correlated(r=-0.756～-0.621,all P＜0.05).Glomerular filtration rate was positively correlated(r=0.695,P=0.010).ROC curve analysis showed that the sensitivity and specificity of circAKT3 in the diagnosis of DM were 75.3%and 71.1%,respectively,and the AUC was 0.792.The sensitivity,specificity and AUC for M-DN were 90.3%,85.7%and 0.875,respectively,and the sensitivity,specificity and the AUC for MS-DN were 88.2%,87.6%and 0.916,respectively.Conclusion The level of serum circAKT3 is significantly down-regulated in DN patients,which can be used as a potential auxiliary diagnostic marker of DN.关键词
糖尿病/糖尿病肾病/环状核糖核酸/蛋白激酶BKey words
diabetes/diabetes nephropathy/circular RNA/protein kinase B分类
医药卫生引用本文复制引用
王晋竹,陶娅,王金帆,蒋娟..2型糖尿病肾病患者外周血差异表达circRNA筛选及circAKT3水平表达与肾纤维化的相关性研究[J].现代检验医学杂志,2025,40(5):46-51,6.基金项目
绵阳市卫生健康委医学科研课题(202320) （202320）
2021年四川省医学(青年创新)科研课题(S215188). （青年创新）
