摘要
Abstract
[Objective]To establish the quality control method of classical Suting Pills.[Method]TLC was used to characterize the identification of fried Perilla frutescens seed and stir-fried Descurainiae Semen in Suting Pills.High performance liquid chromatography(HPLC)was used for quantitative analysis and fingerprinting.The column was InfinttyLab Poroshell 120 EC-C18 column(4.6×150 mm,4 μm),with an injection volume of 8 μL,a column temperature of 30℃,a flow rate of 0.8 mL/min,and a gradient elution with 0.1%phosphoric acid aqueous solution(A)-acetonitrile(B)as the mobile phase(0～6 min,7%～9%B;6～30 min,9%～43%B;30～32 min,43%～90%B;32～40 min,90%B).The fingerprint and the detection wavelength of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside were 254 nm,and rosmarinic acid was 320 nm.The fingerprints of 10 batches of Sufa Pills were collected and evaluated for similarity using the"Chinese Medicine Chromatographic Fingerprint Evaluation System"(2012 Eidition),and the contents of the two index components were determined simultaneously using the HPLC method.[Results]he results of TLC separation were good,the characteristic spots were clearly displayed,and there was no interference in the negative control.The similarity of the fingerprints of 10 batches of Sulphur scape pills was greater than 0.9,and a total of 7 common peaks were identified.The average spiked recoveries of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside and rosmarinic acid were 99.90%and 96.06%,respectively,and the RSD was less than 3%,and the components showed a good linear relationship within a certain injection range.The mass fractions of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside and rosmarinic acid in 10 batches of Suting Pills ranged from 0.081～0.459 and 1.228～1.618 mg/g,respectively.[Conclusion]This method is simple,stable,accurate and reliable,and can be used for the quality control of Suting Pills,as well as laying the foundation for the development of related formulations of Suting Pills.关键词
苏葶丸/质量控制/薄层色谱/指纹图谱/含量测定Key words
Suting Pill/quality control/TLC/fingerprint spectrum/content determination分类
医药卫生
