天津中医药大学学报2025，Vol.44Issue(8)：698-707,10.DOI:10.11656/j.issn.1673-9043.2025.08.05
基于UPLC-QTOF-MS技术分析党参产地加工前后的化学成分差异
A comparative study on chemical constituents diffenrences between fresh and processed samples of Codonopsis Pilosula
摘要
Abstract
[Objective]This study investigates the chemical composition differences between fresh Codonopsis pilosula(Dangshen)medicinal material and its decoction pieces,focusing on the tissue-specific distribution of components and the effects of post-harvest processing on their chemical profile.[Methods]Ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry(UPLC-QTOF-MS)was used to analyze the chemical components of fresh and processed Dangshen.Data were processed using MS-DIAL software and further analyzed through principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)to identify chemical markers distinguishing fresh material from decoction pieces.Additional analyses explored the chemical composition variations across different tissue sections of the decoction pieces,including the periderm,xylem,and phloem.[Results]The results revealed significant chemical composition differences between fresh Dangshen and its decoction pieces.Seventeen differential chemical markers,including alkaloids,organic acids,polyacetylenes,and phenylpropanoids,were identified,each displaying distinct variation patterns.Among these,polyacetylenes exhibited the most notable changes,with a marked enrichment in the xylem of the decoction pieces.[Conclusion]Post-harvest processing was found to have a profound impact on the polyacetylenes,the key active ingredients in Dangshen.The proportion of lobetyolin in the xylem emerged as a critical factor influencing the quality of the decoction pieces.These findings offer valuable insights into optimizing post-harvest processing,understanding quality formation,and establishing quality evaluation criteria for Dangshen medicinal material.关键词
党参/产地加工/UPLC-QTOF-MS/化学成分/质量评价Key words
Codonopsis pilosula/local processing/UPLC-QTOF-MS/chemical constituents/quality evaluation分类
医药卫生引用本文复制引用
何敏,胡佳栋,张晨,邸鹏,唐昭辉,陈万生,邱实..基于UPLC-QTOF-MS技术分析党参产地加工前后的化学成分差异[J].天津中医药大学学报,2025,44(8):698-707,10.基金项目
国家重点研发计划(2022YFC3501700) （2022YFC3501700）
上海市大学生创新创业训练计划项目(202410268245) （202410268245）
上海中医药大学有组织科研项目(2023YZZ02) （2023YZZ02）
中央本级重大增减支项目"名贵中药资源可持续利用能力建设项目"(2060302). （2060302）
