天津中医药大学学报2025，Vol.44Issue(8)：715-721,7.DOI:10.11656/j.issn.1673-9043.2025.08.07
石菖蒲-川芎配伍通过调节Nrf2/HO-1通路改善阿尔茨海默病的作用机制研究
Mechanisms of calamus chinensis-chuanxiong ameliorating Alzheimer's disease by regulating the Nrf2/HO-1 pathway
摘要
Abstract
[Objective]To investigate the effects of water extract of acorus tatarinowii and ligusticum chuanxiong(SCP-CX)on anti-oxidative stress and cognitive function in mice with Alzheimer's disease(AD)induced by D-galactose-sodium nitrite(D-gal—NaNO2).[Methods]Eight-month-old C57BL/6 mice were randomly divided into Control group,Model group,and drug administration group(Donepezil group(0.5 mg/kg),SCP group(1.6 mg/kg),CX group(0.8 mg/kg)and SCP-CX group(1.6 mg/kg vs.0.8 mg/kg),each group with 10 mice.AD model mice were established by subcutaneous injection of D-gal—NaNO2 for 12 consecutive weeks,and the drug was administered by gavage starting after the 4th week of modeling.The learning and spatial memory abilities of mice were evaluated by Morris water maze experiment,the serum levels of glutathione peroxidase(GSH-PX),malondialdehyde(MDA)and superoxide dismutase(SOD)activities of mice were detected by the kit,and the levels of reactive oxygen species(ROS)and β-amyloid1-40(Aβ1-40)in the hippocampal area of mouse brain tissue were determined by ELISA,and the protein expression of nuclear factor-erythroid 2 related factor 2(Nrf2),heme oxygenase 1(HO-1)in mouse brain tissue was detected by Western Blotting.[Results]Compared with the Model group,the escape latency was significantly shorter(P＜0.05)and the number of ascending plateaus increased(P＜0.05)in each dosing group;GSH-Px levels were significantly increased(P＜0.05);SOD activity was significantly decreased(P＜0.05);ROS levels were significantly reduced(P＜0.05);and Aβ1-40 deposition was significantly reduced(P＜0.05).Compared with the Model group,the SCP-CX group could significantly increase Nrf2 and HO-1 protein expression(P＜0.05);compared with the SCP group,the SCP-CX group was able to significantly increase Nrf2 protein expression(P＜0.05).[Conclusion]SCP-CX can improve cognitive dysfunction in AD model mice by modulating the Nrf2/HO-1 signaling pathway to reduce oxidative stress and Aβ deposition in the mouse brain.关键词
石菖蒲/川芎/阿尔茨海默病/氧化应激/Nrf2/HO-1信号通路Key words
calamus/chuanxiong/Alzheimer's disease/oxidative stress/Nrf2/HO-1 signaling pathway分类
医药卫生引用本文复制引用
苏琳琳,梁冰,许海英,郭莉琛,曹玉爽,杜鑫苑,张彤,袁庆,胡利民..石菖蒲-川芎配伍通过调节Nrf2/HO-1通路改善阿尔茨海默病的作用机制研究[J].天津中医药大学学报,2025,44(8):715-721,7.基金项目
天津市科学基金资助项目(21JCYBJC01260). （21JCYBJC01260）
