宿州学院学报2025，Vol.40Issue(6)：15-20,70,7.DOI:10.3969/j.issn.1673-2006.2025.06.003
基于流式细胞术测定王枣子基因组大小
Determination of the Genome Size of Isodon suzhouensis(Wangzaozi)Based on Flow Cytometry
摘要
Abstract
Isodon suzhouensis(Wangzaozi)is a medicinal plant belonging to the Lamiaceae family and is a geogra-phical indication product of Suzhou City.It has been traditionally used in folk medicine to treat various diseases.In this study,aiming to conduct genome sequencing of Isodon suzhouensis and explore genes related to its biosynthesis.A flow cytometry method to detect the genome size of Isodon suzhouensis was established by using Nicotiana benthamiana,Arabidopsis thaliana,and Salvia miltiorrhiza as reference plants.This study also compared the genome size of Isodon suzhouensis to other plants in the Labiaceae family to determine its phylogenetic relationship.The stu-dy suggests that Galbraith is a more suitable extraction solution for isolating nuclei from Isodon suzhouensis compared to OTTO extraction solution.The genome size of Isodon suzhouensis was found to be approximately 430 Mb,which is similar to the genome size of Rabdosia rubescens,indicating a close relationship between the two species.These fin-dings provide valuable insights for future genome sequencing of Isodon suzhouensis.关键词
王枣子/流式细胞术/基因组/冬凌草Key words
Isodon suzhouensis(Wangzaozi)/flow cytometry method/genome/Rabdosia rubescens分类
医药卫生引用本文复制引用
刘发旺,翟科峰,张银,李璐恒..基于流式细胞术测定王枣子基因组大小[J].宿州学院学报,2025,40(6):15-20,70,7.基金项目
安徽省教育厅自然科学研究项目(2024AH051806) （2024AH051806）
宿州学院大学生创新创业训练计划项目(ZCXM24-167 （）
ZCXM24-201) （）
宿州学院非财政资金科研项目(2024xhx196) （2024xhx196）
宿州学院博士科研启动基金项目(2022BSK033) （2022BSK033）
宿州学院教学研究项目(szxy2024jyxm25). （szxy2024jyxm25）
