现代畜牧科技Issue(9)：1-4,4.DOI:10.19369/j.cnki.2095-9737.2025.09.001
犊牛轮状病毒TaqMan荧光定量PCR快速检测方法的建立
Establishment of a Rapid Detection Method for Calf Rotavirus Using TaqMan Fluorescence Quantitative PCR
摘要
Abstract
By analyzing the VP6 gene sequence characteristics of BRV in GenBank,this study successfully optimized specific primers and probes,established standard curves,and comprehensively validated the method's sensitivity,specificity,and reproducibility.The results showed excellent linear correlation between the Ct values of plasmid standards and their concentrations(R2=0.999 5),with a detection limit as low as 2.18×101 copies/μL.Additionally,the method only detected the specific amplification curve of BRV plasmid standards,with no fluorescence signal from negative controls,indicating good specificity.The coefficient of variation was less than 1%,suggesting excellent reproducibility.When applied to clinical samples,the method achieved a positive detection rate of 14.0%for porcine rotavirus infection,compared to 12.0%using conventional PCR methods.This method is suitable for clinical testing.关键词
犊牛/TaqMan/荧光定量PCR/轮状病毒/检测Key words
calf/TaqMan/fluorescence quantitative PCR/rotavirus/test分类
农业科技引用本文复制引用
王小月,代广,董李学,孙月川,常丽云,王睿尧,张子佳..犊牛轮状病毒TaqMan荧光定量PCR快速检测方法的建立[J].现代畜牧科技,2025,(9):1-4,4.基金项目
河北省现代农业产业技术体系建设专项资金(HBCT2023180403) （HBCT2023180403）
唐山市畜禽肠道健康调控基础创新团队(23130234E) （23130234E）
