亚太传统医药2025，Vol.21Issue(9)：13-19,7.DOI:10.11954/ytctyy.202509003
基于b-FGF/Akt/Caspase-9探讨脱管散促进压力性损伤创面愈合机制
To Explore the Machenlsm of Tuoguan Powder in Promoting Pressure Injury Wound Healing Based on b-FGF/Akt/Caspase-9
摘要
Abstract
Objective:To explore the temporal expression and interactions of b-FGF,Akt,and Caspase-9 in pressure ul-cer wound tissues.Methods:The rats were randomly divided into control group(A),model group(B),moist burn oint-ment group(C),Tuoguan powder low(D),medium(E),and high dose groups(F),with 10 rats in each group.The pressure injury model was established by local tissue ischemia-reperfusion mechanism in rats.On days 3,7,and 14 post-induction,the expression of b-FGF mRNA in wound tissue was detected by fluorescence quantitative PCR,the protein expression of b-FGF in wound tissue was detected by Western blot(WB)technique,the content of p-Akts473 in wound tissue was determined by ELISA,and the expression of p-Caspase-9s196 in wound tissue was detected by immunohisto-chemistry.Results:Compared with group A,the expression of b-FGF mRNA,b-FGF in each group was higher than that in group A,and the difference was statistically significant on day 3 and 7(P＜0.05).The expressions of p-AKts473 and p-Caspase-9S196 in each group were higher than those in group A(P＜0.05).Compared with group B,the expres-sion of b-FGF mRNA,b-FGF in groups C,D,E and F was significantly higher on the 3rd and 7th day after administra-tion(P＜0.05),and decreased on the 14th day after administration.The expressions of p-AKts473 and p-caspase-9s196 in groups C,D,E and F were significantly higher than those in group B(P＜0.05).Compared with group C,the expres-sions of b-FGF mRNA,b-FGF,p-AKts473 and p-caspase-9s196 in groups D,E and F were higher than those in group C,and the differences were statistically significant on the third day after administration(P＜0.05).Conclusion:Tuoguan Powder can regulate the expression of b-FGF in the process of pressure injury wound healing to promote wound angio-genesis and avoid scar tissue formation.It can promote the healing of pressure injury wounds in rats by regulating the expressions of p-Akts473 and p-Caspase-9s196,activating PI3K/Akt signaling pathway and inhibiting cell apoptosis.关键词
压力性损伤/脱管散/碱性成纤维细胞生长因子/蛋白激酶B/半胱氨酸天冬氨酸酶-9Key words
Pressure Injury/Tuoguan Powder/Basic Fibroblast Growth Factor/Protein Kinase B/Caspase-9分类
医药卫生引用本文复制引用
战青,张乐乐,杨淑珍,李巧梅,王波..基于b-FGF/Akt/Caspase-9探讨脱管散促进压力性损伤创面愈合机制[J].亚太传统医药,2025,21(9):13-19,7.基金项目
河西学院校长基金青年科研项目(QN2023016) （QN2023016）
