现代畜牧科技Issue(9)：17-20,4.DOI:10.19369/j.cnki.2095-9737.2025.09.005
5种消毒剂对猪繁殖与呼吸综合征病毒核酸荧光定量PCR检测结果的影响
5 Disinfectants to Fluorescence Quantitative PCR of Nucleic Acids for Porcine Reproductive and Respiratory Syndrome Viruses Impact of Test Results
刘喆 1方芬 2陈亮 1康金国 1张忠海1
作者信息
- 1. 上海市浦东新区畜牧水产技术推广中心,上海 201299
- 2. 勃林格殷格翰动物保健(中国)有限公司上海分公司,上海 201323
- 折叠
摘要
Abstract
In order to understand the effect of disinfection of aldehyde preparations(glutaraldehyde),quaternary ammonium salts,and halogen(potassium persulfate)disinfectants on viral nucleic acids,five disinfectants were selected respectively.After diluting the working concentration recommended in the disinfectant instructions,each disinfectant was diluted at 1∶64 and 1∶128 concentrations.The diluted disinfectant acted with the pig breeding and respiratory syndrome virus nucleic acid for 5 minutes,10 minutes,and 15 minutes respectively.The reactants were taken and quantitative PCR was used for detection.The results showed that the most significant effect of glutaraldehyde(A)and potassium bisulate(C3)disinfectants under laboratory conditions on pig reproduction and respiratory syndrome virus nucleic acids,and the PCR test results were both Ct of 40.Other disinfectants have slightly weaker nucleic acid degradation ability of pig breeding and respiratory syndrome viruses,and the detection Ct value has only increased slightly.This study evaluated the destructive ability of five disinfectants to pig reproductive and respiratory syndrome virus nucleic acids,which can provide reference for pig farms when analyzing the effects of disinfectants.关键词
猪繁殖与呼吸综合征/消毒剂/荧光定量PCR/效果Key words
porcine reproductive and respiratory syndrome/disinfectant/fluorescence quantitative PCR/effectiveness分类
农业科技引用本文复制引用
刘喆,方芬,陈亮,康金国,张忠海..5种消毒剂对猪繁殖与呼吸综合征病毒核酸荧光定量PCR检测结果的影响[J].现代畜牧科技,2025,(9):17-20,4.
